Top-down peptidomics of bodily fluids

The naturally occurring peptides, mainly arising from the proteolytic cleavage of larger proteins, play several functions within the body (e. g. antihypertensive, immuno-modulatory, anti-microbial and antiviral, mineral carriers). Their presence or the increase of their concentration could be connected to different pathologies and thereby some peptides could be useful biomarkers for the diagnosis or prognosis of the disease. Peptidome research, particularly within biological fluids, therefore represents one of the most interesting and challenging purposes of proteomics. In this review we describe the current state-of-the-art in peptidomics-based studies of several human bodily fluids (serum, plasma, urine, cerebrospinal fluid, saliva, tears, seminal fluid, vitreous humor, pancreatic juice), emphasizing the contribution of top-down proteomic platforms to the deep structural characterization of natural peptides and their posttranslational modifications.