Biosensors are projected to find many applications, due to the advantages of being highly specific and sensitive, rapid, economical and easy-to-handle for in-field measurements (1,2). As stated by recent reports published by Global Industry Analysts Inc, United States and Europe dominate the global market for biosensors, collectively capturing 69.73% share estimated in 2008, and it is likely to continue to experience steady growth in the years beyond 2009. A main problem of biosensor technology is due to the fact that it requires a specific sensor set-up for each biomediator. We developed, by the synergy of an R&D institution (Italian CNR) and an industrial high tech company (Biosensor Srl), advanced instruments which satisfy fundamental requirements, thanks to the modularity of the measurement cells, being suitable for various biologic materials. In particular, collecting results from many years of experience in biosensor field, we designed and realized an automatic sensoristic platform which combines the most used transduction systems for biosensor: amperometric, to measure an electrochemical current by means of screen-printed-electrodes (SPE) (Fig.1), and optical, to measure an emitted fluorescence signal by means of LEDs and photodiodes (Fig.2) (3), and that is able to accommodate several types of biological sensing elements. We tested the platform with various biologic materials like laccase, tyrosinase and photosynthetic thylakoids. Starting from the most significant experiments on food analysis and environmental monitoring, we realized a protocol of analysis based on the use of a multi-array of mutant strains from the photosynthetic unicellular green alga Chlamydomonas reinhardtii, for the detection of pollutants owning to large classes of chemical compounds (4,5). The resulting platform which employs the two transducers in one system can give additional and/or complementary information about the state of the biologic material, allowing the analyst to obtain more unambiguous results than those obtained applying only one of the two techniques.
A new platform of biosensors contemporaneous measurement of electrogenic and fluorescent biomaterials to support the technical development of most biosensors
Giardi MT;Scognamiglio V;Romagnoli P;
2009
Abstract
Biosensors are projected to find many applications, due to the advantages of being highly specific and sensitive, rapid, economical and easy-to-handle for in-field measurements (1,2). As stated by recent reports published by Global Industry Analysts Inc, United States and Europe dominate the global market for biosensors, collectively capturing 69.73% share estimated in 2008, and it is likely to continue to experience steady growth in the years beyond 2009. A main problem of biosensor technology is due to the fact that it requires a specific sensor set-up for each biomediator. We developed, by the synergy of an R&D institution (Italian CNR) and an industrial high tech company (Biosensor Srl), advanced instruments which satisfy fundamental requirements, thanks to the modularity of the measurement cells, being suitable for various biologic materials. In particular, collecting results from many years of experience in biosensor field, we designed and realized an automatic sensoristic platform which combines the most used transduction systems for biosensor: amperometric, to measure an electrochemical current by means of screen-printed-electrodes (SPE) (Fig.1), and optical, to measure an emitted fluorescence signal by means of LEDs and photodiodes (Fig.2) (3), and that is able to accommodate several types of biological sensing elements. We tested the platform with various biologic materials like laccase, tyrosinase and photosynthetic thylakoids. Starting from the most significant experiments on food analysis and environmental monitoring, we realized a protocol of analysis based on the use of a multi-array of mutant strains from the photosynthetic unicellular green alga Chlamydomonas reinhardtii, for the detection of pollutants owning to large classes of chemical compounds (4,5). The resulting platform which employs the two transducers in one system can give additional and/or complementary information about the state of the biologic material, allowing the analyst to obtain more unambiguous results than those obtained applying only one of the two techniques.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
