Breed identification of animal products is a fundamental tool used to control food quality, add value to regional products and exploit local breeds as well as prevent food adulteration. Myostatin (MSTN) and Melanocortin I Receptor (MC1R) are ideal candidate genes for molecular breed traceability of cattle because of their breed-specific variability. A novel method based on primer extension (SNaPshot) was successfully used for the MSTN and MC1R genotyping of eight bovine breeds. A total of 114 samples from hair bulb and cheese were analyzed. The results obtained allowed the breeds to be identified and results were confirmed by PCR-RFLP and DNA sequencing. The proposed SNaPshot method provides rapid throughput analysis from highly fragmented DNA of processed foods from animals, such as cheese
Cattle breed traceability in meat and cheese using dna polymorphisms detected by the snapshot method
2009
Abstract
Breed identification of animal products is a fundamental tool used to control food quality, add value to regional products and exploit local breeds as well as prevent food adulteration. Myostatin (MSTN) and Melanocortin I Receptor (MC1R) are ideal candidate genes for molecular breed traceability of cattle because of their breed-specific variability. A novel method based on primer extension (SNaPshot) was successfully used for the MSTN and MC1R genotyping of eight bovine breeds. A total of 114 samples from hair bulb and cheese were analyzed. The results obtained allowed the breeds to be identified and results were confirmed by PCR-RFLP and DNA sequencing. The proposed SNaPshot method provides rapid throughput analysis from highly fragmented DNA of processed foods from animals, such as cheeseI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
