High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid–type glutamate receptor clusters on hippocampal neurons. The GluR2 subunits were labeled with the die Alexa 488 and high-resolution infrared micrographs were taken at 6.25 µm. The absence of photobleaching makes this approach suitable for a long-term observation and allows to localize different infrared-absorbing molecules over the complex background of other cell components.

Photobleaching-free infrared near-field microscopy localizes molecules in neurons

2008

Abstract

High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid–type glutamate receptor clusters on hippocampal neurons. The GluR2 subunits were labeled with the die Alexa 488 and high-resolution infrared micrographs were taken at 6.25 µm. The absence of photobleaching makes this approach suitable for a long-term observation and allows to localize different infrared-absorbing molecules over the complex background of other cell components.
2008
Istituto di Struttura della Materia - ISM - Sede Roma Tor Vergata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/23481
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