Esca is the most important disease of the woody tissue of grapevine. Several microorganisms with different role are involved in the infection process where Phaeoacremonium aleophilum and P. chlamydosporum have been identified as pioneering agents. Once this disease is established no effective control can be achieved. The production of a more highly antifungal environment enhancing wood constitutive barriers was attempted by assessing the influence of resveratrol (the most representative wood grapevine stilbene) and its interaction with phosetyl Al and phosphorous acid on the mycelial growth of P. aleophum and P. chlamydosporum, under laboratory and greenhouse conditions. Moreover, the mechanism of inhibitory compounds detoxification by phenol oxidase enzyme tests was investigated. In vitro, resveratrol poorly reduced mycelial growth. On the other hand, the mixture of phosphorous acid and resveratrol proved to be very effective, more than applying these compounds individually. No staining reactions occurred with laccase and peroxidase, except for P. aleophilum that exhibited catalase activity. In vivo trials showed a significant reduction of the necrotic area on treated plants as compared with the unsprayed, inoculated control. These promising preliminary results are discussed.
In vitro studies on the phosphorous acid - Vitis stilbenes interaction, and in vivo phosetyl Al activity towards Phaeoacremonium spp. grapevine wood decay agents
Di Marco S;
1999
Abstract
Esca is the most important disease of the woody tissue of grapevine. Several microorganisms with different role are involved in the infection process where Phaeoacremonium aleophilum and P. chlamydosporum have been identified as pioneering agents. Once this disease is established no effective control can be achieved. The production of a more highly antifungal environment enhancing wood constitutive barriers was attempted by assessing the influence of resveratrol (the most representative wood grapevine stilbene) and its interaction with phosetyl Al and phosphorous acid on the mycelial growth of P. aleophum and P. chlamydosporum, under laboratory and greenhouse conditions. Moreover, the mechanism of inhibitory compounds detoxification by phenol oxidase enzyme tests was investigated. In vitro, resveratrol poorly reduced mycelial growth. On the other hand, the mixture of phosphorous acid and resveratrol proved to be very effective, more than applying these compounds individually. No staining reactions occurred with laccase and peroxidase, except for P. aleophilum that exhibited catalase activity. In vivo trials showed a significant reduction of the necrotic area on treated plants as compared with the unsprayed, inoculated control. These promising preliminary results are discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
