The Salento (Puglia) carbonate aquifer is an important water reservoir which presents an high vulnerability to the wastewater pollution due to numerous vertical fractures of the rock aquifer. An experimental study has been carried out in the Nardò aquifer (Salento region), where the channel Asso outflows rainfall and other waste's drainages in a natural sinkhole (i.e., karst cavity). The study for qualitative and quantitative viral estimations, was supported by a groundwater monitoring in wells carried out by CNR-IRSA during winter 2002. The collected water samples were analyzed for viral contamination tests by using the culture cell method at Bari University and the RT-nested PCR at Pisa University. Seventeen water samples from selected wells located downgradient the sinkhole with respect to groundwater flow, resulted positive at the RT-PCR and were analysed by cell methods in order to perform differential viral analyses. Three cell lines (BGM, Hep-2 and L20B) were seeded with water samples following two protocols: A and B. Following the protocol A, the water samples were only subjected to antibiotics pretreatment, whereas following the protocol B, the samples were also 10% chloroform treated. The two combined virological methods revealed ground water contamination by Poliovirus 2 (10-5.5CCID50/25mL) and Echovirus 11 (10-5 CCID50/25 mL) in two samples from a well 500 m from the sinkhole and by Poliovirus 3 (10-5CCID50/25mL) in sampled water from a well 3000 m from contamination. The results deserve considerations and suggest severe virological controls of the water from domestic wells water of the Salento.

Entero viruses in domestic wells of the Salento groundwater (Southern Italy)

MASCIOPINTO C;
2004-01-01

Abstract

The Salento (Puglia) carbonate aquifer is an important water reservoir which presents an high vulnerability to the wastewater pollution due to numerous vertical fractures of the rock aquifer. An experimental study has been carried out in the Nardò aquifer (Salento region), where the channel Asso outflows rainfall and other waste's drainages in a natural sinkhole (i.e., karst cavity). The study for qualitative and quantitative viral estimations, was supported by a groundwater monitoring in wells carried out by CNR-IRSA during winter 2002. The collected water samples were analyzed for viral contamination tests by using the culture cell method at Bari University and the RT-nested PCR at Pisa University. Seventeen water samples from selected wells located downgradient the sinkhole with respect to groundwater flow, resulted positive at the RT-PCR and were analysed by cell methods in order to perform differential viral analyses. Three cell lines (BGM, Hep-2 and L20B) were seeded with water samples following two protocols: A and B. Following the protocol A, the water samples were only subjected to antibiotics pretreatment, whereas following the protocol B, the samples were also 10% chloroform treated. The two combined virological methods revealed ground water contamination by Poliovirus 2 (10-5.5CCID50/25mL) and Echovirus 11 (10-5 CCID50/25 mL) in two samples from a well 500 m from the sinkhole and by Poliovirus 3 (10-5CCID50/25mL) in sampled water from a well 3000 m from contamination. The results deserve considerations and suggest severe virological controls of the water from domestic wells water of the Salento.
2004
Istituto di Ricerca Sulle Acque - IRSA
Natural water quality
Wells
Waterborne disease outbreaks
Pathogenic microorganisms detection
Natural water disinfection
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/235793
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