Microscale isolation technique for quantitative GC-MS analysis of indole-3-acetic acid from cherry (Prunus cerasus L.)

A rapid minicolumn technique is described that has been used for the isolation and mass spectral quantification of indole-3-acetic acid from cherry, a difficult to analyze tissue. The method used the resin XAD-7 to trap impurities. Samples were further purified on an amino minicolumn and then by high-performance liquid chromatography on a C18 column. After methylation, the samples were analyzed by selected ion monitoring gas chromatography-mass spectrometry. 13C6indole-3-acetic acid was employed as an internal standard for isotope dilution calculations.