The thermophilic and thermostable family B DNA polymerase from the archaeon Sulfolobus solfataricus (M-r of about 100 kDa) has been crystallized by the hanging-drop vapour-diffusion method at 294 K using ammonium sulfate as precipitant. The crystals belong to the monoclinic space group C2 with cell dimensions a = 187.4. b = 68.5, c = 125.8 Angstrom and beta = 107.8 degrees and diffract up to 2.7 Angstrom resolution on a rotating-anode X-ray source. Native data have been collected at 100 K. A heavy-atom derivative search is in progress.
Crystallization and preliminary X-ray diffraction studies of DNA polymerase from the thermophilic archaeon Sulfolobus solfataricus
Pisani FM;Savino C;Rossi M;
1998
Abstract
The thermophilic and thermostable family B DNA polymerase from the archaeon Sulfolobus solfataricus (M-r of about 100 kDa) has been crystallized by the hanging-drop vapour-diffusion method at 294 K using ammonium sulfate as precipitant. The crystals belong to the monoclinic space group C2 with cell dimensions a = 187.4. b = 68.5, c = 125.8 Angstrom and beta = 107.8 degrees and diffract up to 2.7 Angstrom resolution on a rotating-anode X-ray source. Native data have been collected at 100 K. A heavy-atom derivative search is in progress.File in questo prodotto:
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