An optofluidic water-jet waveguide on chip for fluorescence analysis is presented. A high-speed water stream produced by means of a microchannel acts at the same time as the solution to analyze and as the collecting optical waveguide. The absence of solid walls and the very optically smooth surface of the liquid stream permits a strong increase of the signal-to-noise ratio. The device layout allows a self-alignment direct coupling of a water-jet waveguide with a multimode optical fiber connected to the detector. The performances of the integrated system are tested on Cy5 water solutions. For a 4.5mm long water-jet waveguide, the measured limit of detection (LOD) is 2.56nM and filter-free detection is possible with an LOD of 6.11nM.
Optofluidic jet waveguide for laser-induced fluorescence spectroscopy
Persichetti G;Testa G;Bernini R
2012
Abstract
An optofluidic water-jet waveguide on chip for fluorescence analysis is presented. A high-speed water stream produced by means of a microchannel acts at the same time as the solution to analyze and as the collecting optical waveguide. The absence of solid walls and the very optically smooth surface of the liquid stream permits a strong increase of the signal-to-noise ratio. The device layout allows a self-alignment direct coupling of a water-jet waveguide with a multimode optical fiber connected to the detector. The performances of the integrated system are tested on Cy5 water solutions. For a 4.5mm long water-jet waveguide, the measured limit of detection (LOD) is 2.56nM and filter-free detection is possible with an LOD of 6.11nM.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
