cis-Diamminedichloroplatinum(II) (cisplatin) is able to interact with human superoxide dismutase (hSOD1) in the disulfide oxidized apo form with a dissociation constant of 37 ± 3 ?M through binding cysteine 111 (Cys111) located at the edge of the subunit interface. It also binds to Cu2-Zn2 and Zn2-Zn2 forms of hSOD1. Cisplatin inhibits aggregation of demetalated oxidized hSOD1, and it is further able to dissolve and monomerize oxidized hSOD1 oligomers in vitro and in cell, thus indicating its potential as a leading compound for amyotrophic lateral sclerosis.
Interaction of Cisplatin with Human Superoxide Dismutase
Angela Trapananti;Mauro Cozzolino;
2012
Abstract
cis-Diamminedichloroplatinum(II) (cisplatin) is able to interact with human superoxide dismutase (hSOD1) in the disulfide oxidized apo form with a dissociation constant of 37 ± 3 ?M through binding cysteine 111 (Cys111) located at the edge of the subunit interface. It also binds to Cu2-Zn2 and Zn2-Zn2 forms of hSOD1. Cisplatin inhibits aggregation of demetalated oxidized hSOD1, and it is further able to dissolve and monomerize oxidized hSOD1 oligomers in vitro and in cell, thus indicating its potential as a leading compound for amyotrophic lateral sclerosis.File in questo prodotto:
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