The immobilization of brain dopamine receptors (primary targets in the treatment of schizophrenia, Parkinson's disease, and Huntington's chorea) on a solid support can represent an appealing strategy for the realization of biosensors for dopamine and dopaminergic drugs. Our final goal is the realization of an optical biosensor that allows parallel quantitative analysis, with different receptors, of neurotransmitters and related drugs. In our scheme, the active layer is constituted by dopaminergic receptors immobilized in biomimetic membranes tethered to a sensor surface. In order to preserve the biological activity of the neuroreceptors, the fabrication is carried out by soft lithography methods. In this report, we have characterised the active layer by scanning probe techniques, in order to test its quality and integrity, and by optical experiments involving the signal emitted by specific fluorescent ligands.

Characterization of a tethering system for biosensor applications

Laura Blasi;Dario Pisignano;Antonia Mallardi;Giuseppe Maruccio;Giuseppe Ciccarella;Francesco Calabi;Rosaria Rinaldi
2005

Abstract

The immobilization of brain dopamine receptors (primary targets in the treatment of schizophrenia, Parkinson's disease, and Huntington's chorea) on a solid support can represent an appealing strategy for the realization of biosensors for dopamine and dopaminergic drugs. Our final goal is the realization of an optical biosensor that allows parallel quantitative analysis, with different receptors, of neurotransmitters and related drugs. In our scheme, the active layer is constituted by dopaminergic receptors immobilized in biomimetic membranes tethered to a sensor surface. In order to preserve the biological activity of the neuroreceptors, the fabrication is carried out by soft lithography methods. In this report, we have characterised the active layer by scanning probe techniques, in order to test its quality and integrity, and by optical experiments involving the signal emitted by specific fluorescent ligands.
2005
Istituto di Nanotecnologia - NANOTEC
INFM
0976798506
Dopaminergic receptors
Fluorescence spectroscopy
Scanning probes microscopy
Tethered bilayer
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/246
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