A 659 bp cDNA clone** coding for an allergen of Pj pollen has been isolated from a lambda gt 11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par j I since it reacted with 95% of the sera from Pj-allergic patients (n = 22) and with two Par j I-specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.
cDNA cloning, expression and primary structure of Parj 1, a major allergen of Parietaria judaica pollen.
1994
Abstract
A 659 bp cDNA clone** coding for an allergen of Pj pollen has been isolated from a lambda gt 11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par j I since it reacted with 95% of the sera from Pj-allergic patients (n = 22) and with two Par j I-specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
