The carob tree (Ceratonia siliqua), is a typical Mediterranean tree with high hardiness and adaptability to the climatic conditions of marginal and sub-marginal areas. These characteristics, together with it ability to preserve and enrich soil fertility and the economic value of its products (used in food, chemicals, cosmetics, processing etc.) make this species not only a biological tool to counteract processes of erosion and desertification, but also a challenge for development of these areas. However the large-scale cultivation of the carob tree is limited by the traditional methods of propagation that fail to meet the growing demand for carob plants with valuable agro-economical characteristics. This study aimed to develop a more performing protocol for carob tree micropropagation using nodal explants taken from juvenile (young germination) or (female adult tree) plant material. Effectiveness of combination of different concentrations of benzylaminopurine (BAP 0, 0.2, 0.5, 1 and 3 mg/L) and indole butyric acid (IBA 0, 0.2, 0.5, 1 and 3 mg/L) in stimulating the reactivity and proliferation of plant tissue have been assessed. Obtained data have shown that Augmentin and tetracycline were the more effective antibiotics in obtaining in vitro healthy plant material. However, plant tissue proliferation was negatively affected by these antibiotics (decrease of elongation, and number of leaves and internodes). Strong bud initiation was obtained with the juvenile material on all tested hormonal combinations, whereas adult material shows a low bud initiation with a maximum of budding for 1 mg/L and 3 mg/L BAP concentrations. In addition, shoot growth assessed following buds extension and leaves number showed that auxin concentration is the most discriminating factor. The best shoot growth was obtained with 0.5 mg/L of IBA combined to 0.2 mg/L of BAP. Higher IBA concentrations caused a decrease in shoot growth while change of BAP concentration did not significant affect shoot growth
The In Vitro Responses of Nodal Explants to Benzylaminopurine and Indole Butyric Acid Combinations During Carob Tree (Ceratonia siliqua) Micropropagation
Cavallaro V;
2013
Abstract
The carob tree (Ceratonia siliqua), is a typical Mediterranean tree with high hardiness and adaptability to the climatic conditions of marginal and sub-marginal areas. These characteristics, together with it ability to preserve and enrich soil fertility and the economic value of its products (used in food, chemicals, cosmetics, processing etc.) make this species not only a biological tool to counteract processes of erosion and desertification, but also a challenge for development of these areas. However the large-scale cultivation of the carob tree is limited by the traditional methods of propagation that fail to meet the growing demand for carob plants with valuable agro-economical characteristics. This study aimed to develop a more performing protocol for carob tree micropropagation using nodal explants taken from juvenile (young germination) or (female adult tree) plant material. Effectiveness of combination of different concentrations of benzylaminopurine (BAP 0, 0.2, 0.5, 1 and 3 mg/L) and indole butyric acid (IBA 0, 0.2, 0.5, 1 and 3 mg/L) in stimulating the reactivity and proliferation of plant tissue have been assessed. Obtained data have shown that Augmentin and tetracycline were the more effective antibiotics in obtaining in vitro healthy plant material. However, plant tissue proliferation was negatively affected by these antibiotics (decrease of elongation, and number of leaves and internodes). Strong bud initiation was obtained with the juvenile material on all tested hormonal combinations, whereas adult material shows a low bud initiation with a maximum of budding for 1 mg/L and 3 mg/L BAP concentrations. In addition, shoot growth assessed following buds extension and leaves number showed that auxin concentration is the most discriminating factor. The best shoot growth was obtained with 0.5 mg/L of IBA combined to 0.2 mg/L of BAP. Higher IBA concentrations caused a decrease in shoot growth while change of BAP concentration did not significant affect shoot growthI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
