The IVV-CNR Unit of Grugliasco (TO) has selected and registered in the National Grapevine Cultivar Catalogue more than 100 clones belonging to 25 cultivars of north-west Italy. The primary sources of these clones are preserved and pre-multiplicated by the Grapevine Premultiplication Centre of Piedmont (CEPREMAVI), based in Alba (CN) and managed by Vivalb on behalf of the Piemonte Region. Premultiplication supplies the commercial nurseries with propagation material of the "basic" category, i.e. selected and virus-free. In the 2013 campaign CEPREMAVI produced more than 37000 "basic" grafted rootlings. The healthy primary sources, according with official regulations, are kept individually potted inside a screen-house, where each clone is represented by three plants (on its own roots). The isolation from the soil and from the outside, thanks to a double insect-proof net, safeguards the original sanitary status of the clones from both virus and phytoplasma. The "initial material" collected from the primary sources is then used to establish the mother plant vineyards (MPV) suitable for the production of 'basic' material. In recent times, due to the spread of phytoplasma diseases (Flavescence dorée and Bois noir), the CEPREMAVI has set a new approach in the production of clonal "basic" material, introducing the complete isolation from ground (except roots) and the outside also for the MPV. The attempt is to prevent at the highest level the propagated material from any possible virus and phytoplasma infection. The new technique consists in planting the MPV inside insect-proof tunnels provided with a double room entrance and with the complete plastic mulching of the ground. Each plant row is isolated from the others. Along the rows a metallic structure is designed to support the net coverage as well as the wire sets to uphold plant canopy. In the upper part of tunnels a plastic pipe runs with sprinklers for fungicide and insecticide sprays. Vineyard management (i.e. the entry of workers inside the tunnels) is limited to green pruning and to routine sanitary inspections. During winter time the nets are removed from supports and rolled up on the ground near the rows.In order to maintain the optimal sanitary status along the pre-multiplication steps, the woody propagation materials collected from the covered MPV are processed by hot water treatment at 50°C x 45' before grafting. In addition, since the campaign 2013-14, the traditional open-field nursery will be eliminated. After the callusing period, the grafted cuttings will be potted (biodegradable pot) and maintained around 20 days in a green-house, then transferred under a wide tunnel ground-mulched and covered by an insect-proof net, where they will complete the seasonal growth until fall. Thanks to the new technique, the CEPREMAVI will be able to produce "basic" propagation material with the maximum degree of protection from virus and phytoplasma infection.
Innovative techniques for pre-multiplication of grapevine clones: the CEPREMAVI experience
Mannini F;
2013
Abstract
The IVV-CNR Unit of Grugliasco (TO) has selected and registered in the National Grapevine Cultivar Catalogue more than 100 clones belonging to 25 cultivars of north-west Italy. The primary sources of these clones are preserved and pre-multiplicated by the Grapevine Premultiplication Centre of Piedmont (CEPREMAVI), based in Alba (CN) and managed by Vivalb on behalf of the Piemonte Region. Premultiplication supplies the commercial nurseries with propagation material of the "basic" category, i.e. selected and virus-free. In the 2013 campaign CEPREMAVI produced more than 37000 "basic" grafted rootlings. The healthy primary sources, according with official regulations, are kept individually potted inside a screen-house, where each clone is represented by three plants (on its own roots). The isolation from the soil and from the outside, thanks to a double insect-proof net, safeguards the original sanitary status of the clones from both virus and phytoplasma. The "initial material" collected from the primary sources is then used to establish the mother plant vineyards (MPV) suitable for the production of 'basic' material. In recent times, due to the spread of phytoplasma diseases (Flavescence dorée and Bois noir), the CEPREMAVI has set a new approach in the production of clonal "basic" material, introducing the complete isolation from ground (except roots) and the outside also for the MPV. The attempt is to prevent at the highest level the propagated material from any possible virus and phytoplasma infection. The new technique consists in planting the MPV inside insect-proof tunnels provided with a double room entrance and with the complete plastic mulching of the ground. Each plant row is isolated from the others. Along the rows a metallic structure is designed to support the net coverage as well as the wire sets to uphold plant canopy. In the upper part of tunnels a plastic pipe runs with sprinklers for fungicide and insecticide sprays. Vineyard management (i.e. the entry of workers inside the tunnels) is limited to green pruning and to routine sanitary inspections. During winter time the nets are removed from supports and rolled up on the ground near the rows.In order to maintain the optimal sanitary status along the pre-multiplication steps, the woody propagation materials collected from the covered MPV are processed by hot water treatment at 50°C x 45' before grafting. In addition, since the campaign 2013-14, the traditional open-field nursery will be eliminated. After the callusing period, the grafted cuttings will be potted (biodegradable pot) and maintained around 20 days in a green-house, then transferred under a wide tunnel ground-mulched and covered by an insect-proof net, where they will complete the seasonal growth until fall. Thanks to the new technique, the CEPREMAVI will be able to produce "basic" propagation material with the maximum degree of protection from virus and phytoplasma infection.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
