Background: Several types of cell death can induce the activation of the immune system by releasing factors of damage. Aim: To identify different cell death modalities using U87 glioblastoma cell line after radio-chemotherapy treatments by analyzing the expression of HSP70 after gamma-ray irradiation and temozolomide treatment. Materials and Methods: U87 cell line was irradiated with 2, 4, 8, 10 and 20 Gy, treated with 200 mu M of temozolomide, or subjected to combined treatments of these. Results: Forty-eight hours after treatment, apoptosis was more prevalent than necrosis, especially after gamma-radiation with a somewhat dose-dependent trend. Temozolomide did not seem to induce significant modifications of the pattern of cell death and protein expression. Temozolomide-induced cell death was lower or equal to that obtained with high dose radiotherapy. A low synergism between the two treatments was observed. Conclusion: This cell line showed a marked radioresistance to conventional clinical doses, showing attempts to repair the induced damage, while temozolomide treatment showed no cytotoxic effects.
Cell Death Forms and HSP70 Expression in U87 Cells after Ionizing Radiation and/or Chemotherapy
Mazzini Giuliano;
2011
Abstract
Background: Several types of cell death can induce the activation of the immune system by releasing factors of damage. Aim: To identify different cell death modalities using U87 glioblastoma cell line after radio-chemotherapy treatments by analyzing the expression of HSP70 after gamma-ray irradiation and temozolomide treatment. Materials and Methods: U87 cell line was irradiated with 2, 4, 8, 10 and 20 Gy, treated with 200 mu M of temozolomide, or subjected to combined treatments of these. Results: Forty-eight hours after treatment, apoptosis was more prevalent than necrosis, especially after gamma-radiation with a somewhat dose-dependent trend. Temozolomide did not seem to induce significant modifications of the pattern of cell death and protein expression. Temozolomide-induced cell death was lower or equal to that obtained with high dose radiotherapy. A low synergism between the two treatments was observed. Conclusion: This cell line showed a marked radioresistance to conventional clinical doses, showing attempts to repair the induced damage, while temozolomide treatment showed no cytotoxic effects.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
