The objective of the work was to develop a suitable method for covalent immobilization of active biomolecules on hydrophobic membrane. Polyvinylidene fluoride (PVDF) is advantageous over other membrane materials due to its high mechanical strength and excellent chemical resistance. While many information about hydrophilized PVDF is present in the literature, little information on native PVDF functionalization is available. In fact its native structure does not have sites for biomolecules covalent immobilization in mild condition. In the present work surface modification of native PVDF flat-sheet membranes was performed by the wet chemical strategy using 1, 5-diamino-2-methylpentane (DAMP), under basic condition, as carrier of amino groups. Afterwards, these groups were activated with glutaraldehyde (GA) to which biomolecules were attached by covalent bond in mild conditions. DAMP concentration, reaction temperature, and reaction time were studied in order to tune the degree of modification as well as the loading capacity of the functionalized membrane. Modified and native PVDF membranes were characterized, by Fourier transform infrared spectroscopy (FT-IR), ninhydrin test, scanning electron microscopy (SEM) and mechanical resistance test. Two model proteins having different properties (BSA and Lipase) were selected for immobilization test on the modified membranes. Compared with an unmodified PVDF membrane, higher binding capacities were detected for both biomolecules.
PVDF membrane biofunctionalization by chemical grafting
Vitola G;Mazzei R;Fontananova E;Giorno L
2015
Abstract
The objective of the work was to develop a suitable method for covalent immobilization of active biomolecules on hydrophobic membrane. Polyvinylidene fluoride (PVDF) is advantageous over other membrane materials due to its high mechanical strength and excellent chemical resistance. While many information about hydrophilized PVDF is present in the literature, little information on native PVDF functionalization is available. In fact its native structure does not have sites for biomolecules covalent immobilization in mild condition. In the present work surface modification of native PVDF flat-sheet membranes was performed by the wet chemical strategy using 1, 5-diamino-2-methylpentane (DAMP), under basic condition, as carrier of amino groups. Afterwards, these groups were activated with glutaraldehyde (GA) to which biomolecules were attached by covalent bond in mild conditions. DAMP concentration, reaction temperature, and reaction time were studied in order to tune the degree of modification as well as the loading capacity of the functionalized membrane. Modified and native PVDF membranes were characterized, by Fourier transform infrared spectroscopy (FT-IR), ninhydrin test, scanning electron microscopy (SEM) and mechanical resistance test. Two model proteins having different properties (BSA and Lipase) were selected for immobilization test on the modified membranes. Compared with an unmodified PVDF membrane, higher binding capacities were detected for both biomolecules.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
