Liquid chromatography in coupling with high-resolution ESI-LTQ-Orbitrap mass spectrometry was applied for a proteomic study of pediatric pilocytic astrocytoma brain tumor intracystic fluid by an integrated top-down/bottom-up platform. Both of the proteomic strategies resulted comple-mentary and support each other in contributing to a wide characterization of the protein and peptide content of the tumorfluid. Top-down approach allowed to identify several proteins and peptides involved in different biological activities together with the characterization of interesting proteoforms such as fibrinopeptide A and its truncated form,fibrinopeptide B, complement C3f fragments,?-thymosin peptides, ubiquitin,several apolipoproteins belonging to A and C families,apolipoprotein J and D, and cystatin C. Of particular interest resulted the identification of a N-terminal truncated cystatin C proteoform, likely involved in immune response mechanism modulations and the identification of oxidized and glycosylated apolipoproteins including disulfie bridge dimeric forms. The bottom-up approach confirmed some of the experimental datafindingstogether with adding the characterization of high-molecular-mass proteins in the samples. These data could contribute toelucidate the molecular mechanisms involved in onset and progression of the disease and cyst development.
Proteomic Study of Pilocytic Astrocytoma Pediatric Brain Tumor Intracystic Fluid
M Castagnola;
2014
Abstract
Liquid chromatography in coupling with high-resolution ESI-LTQ-Orbitrap mass spectrometry was applied for a proteomic study of pediatric pilocytic astrocytoma brain tumor intracystic fluid by an integrated top-down/bottom-up platform. Both of the proteomic strategies resulted comple-mentary and support each other in contributing to a wide characterization of the protein and peptide content of the tumorfluid. Top-down approach allowed to identify several proteins and peptides involved in different biological activities together with the characterization of interesting proteoforms such as fibrinopeptide A and its truncated form,fibrinopeptide B, complement C3f fragments,?-thymosin peptides, ubiquitin,several apolipoproteins belonging to A and C families,apolipoprotein J and D, and cystatin C. Of particular interest resulted the identification of a N-terminal truncated cystatin C proteoform, likely involved in immune response mechanism modulations and the identification of oxidized and glycosylated apolipoproteins including disulfie bridge dimeric forms. The bottom-up approach confirmed some of the experimental datafindingstogether with adding the characterization of high-molecular-mass proteins in the samples. These data could contribute toelucidate the molecular mechanisms involved in onset and progression of the disease and cyst development.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
