Two methods for monolayer immobilization of photosystem II (PSII) isolated from thermophilic cyanobacteria Synechococcus elongatus and prospects for its use as a biosensor for detection of herbicides are reported: (i) immobilization based on recombinant (HiS)(6)-tagged PSII coupled with nickel-nitrilotriacetic acid (Ni-NTA) chelator monolayer on An electrode. (ii) immobilization based on the natural PSII coupled with the protein A-anti-DI antibody modified Au electrode. Here, Cysteamine (CYS)-self-assembled monolayer (SAM)-(NTA)-PSII monolayers were compared with traditional bovine serum albumin (BSA)-glutaraldehyde (GA)-PSII crosslinked get matrix and better performances of the derived electro-chemical biosensors were pointed out. Better diffusion of inhibitors and mediators resulted in improved sensitivity, velocity of the response, and lower 150 for herbicides.
Monolayers of natural and recombinant photosystem II on gold electrodes - Potentials for use as biosensors for detection of herbicides
Pilloton R
2004
Abstract
Two methods for monolayer immobilization of photosystem II (PSII) isolated from thermophilic cyanobacteria Synechococcus elongatus and prospects for its use as a biosensor for detection of herbicides are reported: (i) immobilization based on recombinant (HiS)(6)-tagged PSII coupled with nickel-nitrilotriacetic acid (Ni-NTA) chelator monolayer on An electrode. (ii) immobilization based on the natural PSII coupled with the protein A-anti-DI antibody modified Au electrode. Here, Cysteamine (CYS)-self-assembled monolayer (SAM)-(NTA)-PSII monolayers were compared with traditional bovine serum albumin (BSA)-glutaraldehyde (GA)-PSII crosslinked get matrix and better performances of the derived electro-chemical biosensors were pointed out. Better diffusion of inhibitors and mediators resulted in improved sensitivity, velocity of the response, and lower 150 for herbicides.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
