A primary therapeutic goal in Alzheimers disease (AD) is to reduce the quantity of amyloid b protein (A b) present in the brain. To develop an effective, safe system for vaccination against Alzheimers disease, the plant virus Cucumber mosaic virus (CMV) was engineered genetically to express A b -derived fragments that stimulate mainly humoral immune responses. Six chimeric constructs, bearing the A b 115 or the A b 415 sequence in positions 248, 392 or 529 of the CMV coat protein (CP) gene, were created. Viral products proved to be able to replicate in their natural host. However, only chimeric A b 115-CMVs were detected by A b 142 antiserum in Western blot analysis. Experimental evidence of Immunoelectron microscopy revealed a complete decoration of A b 115-CMV248 and A b 15-CMV392 following incubation with either anti-A b 115 or anti-A b 142 polyclonal antibodies. These two chimeric CMVs appear to be endowed with features making them possible candidates for vaccination against Alzheimers disease.
Cucumber mosaic virus as the expression system for a potential vaccine against Alzheimer's disease
De Stradis A;
2010
Abstract
A primary therapeutic goal in Alzheimers disease (AD) is to reduce the quantity of amyloid b protein (A b) present in the brain. To develop an effective, safe system for vaccination against Alzheimers disease, the plant virus Cucumber mosaic virus (CMV) was engineered genetically to express A b -derived fragments that stimulate mainly humoral immune responses. Six chimeric constructs, bearing the A b 115 or the A b 415 sequence in positions 248, 392 or 529 of the CMV coat protein (CP) gene, were created. Viral products proved to be able to replicate in their natural host. However, only chimeric A b 115-CMVs were detected by A b 142 antiserum in Western blot analysis. Experimental evidence of Immunoelectron microscopy revealed a complete decoration of A b 115-CMV248 and A b 15-CMV392 following incubation with either anti-A b 115 or anti-A b 142 polyclonal antibodies. These two chimeric CMVs appear to be endowed with features making them possible candidates for vaccination against Alzheimers disease.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.