The presence of Hop stunt viroid (HSVd) was detected using RT-PCR and Northern blot hybridization in five of 60 samples from symptomless mulberry trees (Morus alba) collected in Italian and Lebanese orchards in July 2010. Infection levels were c. 10% in Lebanese and 8% in Italian samples. Nucleotide alignments showed that sequences of the mulberry HSVd isolates shared 95-96% identity with those of the same viroid occurring elsewhere. In a phylogenetic tree, mulberry HSVd isolates clustered together with those of HSVd-citrus, regardless of their geographical origin. This is the first report of infection in mulberry trees by HSVd.
Occurrence of Hop Stunt Viroid in Mulberry (Morus alba) in Lebanon and Italy
Navarro B
2012
Abstract
The presence of Hop stunt viroid (HSVd) was detected using RT-PCR and Northern blot hybridization in five of 60 samples from symptomless mulberry trees (Morus alba) collected in Italian and Lebanese orchards in July 2010. Infection levels were c. 10% in Lebanese and 8% in Italian samples. Nucleotide alignments showed that sequences of the mulberry HSVd isolates shared 95-96% identity with those of the same viroid occurring elsewhere. In a phylogenetic tree, mulberry HSVd isolates clustered together with those of HSVd-citrus, regardless of their geographical origin. This is the first report of infection in mulberry trees by HSVd.File in questo prodotto:
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