Le mutant gem2ts du bactériophage Mu induit le synchronisme des divisions chez les bactéries qui survivent à l'infection. Dans certaines conditions, l'induction du synchronisme peut être toutefois observée chez la totalité des bactéries infectées. C'est ce qui se produit lorsque l'on infecte des mutants hic, une classe particulière d'allèles du gène gyrB. Chez de telles bactéries, il n'y a ni intégration ni replication de l'ADN de Mu, aussi bien pour le phage sauvage que pour le mutant Mu gem2ts, tandis que l'expression des gènes (y compris le gène A qui code pour la transposase) semble être tout à fait normale. Ces données indiquent que le mécanisme à la base de l'induction de la synchronisation par Mu gem2ts ne requiert ni l'intégration ni la replication de l'ADN de Mu.
The gem2ts mutant of bacteriophage Mu induced synchrony of cell division on bacteria surviving infection. Induction of synchronous growth could also be observed as a response to the entire infected bacterial population, as in the case of infection of hic mutants, a peculiar class of gyrB alleles. After Mu wild-type or Mu gem2ts infection of hic mutants, there was a lack of viral DNA integration and replication, while phage gene expression (including that of A gene, coding for the transposase) seemed to be quite normal. These data indicate that the mechanism of bacterial synchronization induced by Mu gem2ts does not require integration nor replication of the phage DNA.
Mu gem2ts DNA integration is not necessary for induction of synchrony of cell division in Escherichia coli K12.
Ghelardini P
1997
Abstract
The gem2ts mutant of bacteriophage Mu induced synchrony of cell division on bacteria surviving infection. Induction of synchronous growth could also be observed as a response to the entire infected bacterial population, as in the case of infection of hic mutants, a peculiar class of gyrB alleles. After Mu wild-type or Mu gem2ts infection of hic mutants, there was a lack of viral DNA integration and replication, while phage gene expression (including that of A gene, coding for the transposase) seemed to be quite normal. These data indicate that the mechanism of bacterial synchronization induced by Mu gem2ts does not require integration nor replication of the phage DNA.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.


