INTRODUCTION: It has been suggested that stromal cells (StC) from early foetal tissues retain a wider differentiation potential compared to adult StC consequence of their developmental origin from embryonic tissues. Aimed at investigate this aspect, a series of in vitro experiments were performed with StC isolated from first trimester human chorionic villi (CVStC). StC from human adult bone marrow were used as control. METHODS AND RESULTS: Chorionic villi stromal cells (CVStC) were isolated from tissue samples by enzymatic digestion and plastic adhesion. CVStC were then cultured in: (1) standard mesenchymal medium (MM) or (2) AmniomaxII® (AM), a medium specifically designed to grow amnion-derived cells during prenatal diagnostic procedures. CVStC cultured in MM or AM presented typical stromal morphology and immunophenotype, were negative for pluripotency factors including Nanog, Oct-4 and Sox-2, lacked detectable telomerase activity and exhibited high genomic stability. In AM, however, CVStC proliferated faster compared to BMStC or CVStC kept in MM. During differentiation, CVStC were less efficient than BMStC acquiring adipocyte and osteocyte features, while cardiomyogenic conversion occurred at low efficiency in both cell types. Surprisingly, after exposure to the endothelium-specific medium EGM-2, AM-cultured CVStC expressed high levels of VEGF-receptor 2 (VEGFR2), formed capillary-like structures and uptaked acetylated LDL. Mechanistically, a reduced CVStC expression of anti-angiogenic microRNAs including miR-21, -221 and -222 has been found associated to this process. CONCLUSIONS: Although of foetal origin, CVStC exhibited a restricted plasticity, similar to that of adult stromal cells and were preferentially pro-angiogenic. CVStC could be a promising biological source suitable for cell therapy applications in vascular diseases.
Mesenchymal Stromal Cells from Human Chorionic Villi Are Preferentially Committed to Endothelial Differentiation.
Antonella Farsetti;
2011
Abstract
INTRODUCTION: It has been suggested that stromal cells (StC) from early foetal tissues retain a wider differentiation potential compared to adult StC consequence of their developmental origin from embryonic tissues. Aimed at investigate this aspect, a series of in vitro experiments were performed with StC isolated from first trimester human chorionic villi (CVStC). StC from human adult bone marrow were used as control. METHODS AND RESULTS: Chorionic villi stromal cells (CVStC) were isolated from tissue samples by enzymatic digestion and plastic adhesion. CVStC were then cultured in: (1) standard mesenchymal medium (MM) or (2) AmniomaxII® (AM), a medium specifically designed to grow amnion-derived cells during prenatal diagnostic procedures. CVStC cultured in MM or AM presented typical stromal morphology and immunophenotype, were negative for pluripotency factors including Nanog, Oct-4 and Sox-2, lacked detectable telomerase activity and exhibited high genomic stability. In AM, however, CVStC proliferated faster compared to BMStC or CVStC kept in MM. During differentiation, CVStC were less efficient than BMStC acquiring adipocyte and osteocyte features, while cardiomyogenic conversion occurred at low efficiency in both cell types. Surprisingly, after exposure to the endothelium-specific medium EGM-2, AM-cultured CVStC expressed high levels of VEGF-receptor 2 (VEGFR2), formed capillary-like structures and uptaked acetylated LDL. Mechanistically, a reduced CVStC expression of anti-angiogenic microRNAs including miR-21, -221 and -222 has been found associated to this process. CONCLUSIONS: Although of foetal origin, CVStC exhibited a restricted plasticity, similar to that of adult stromal cells and were preferentially pro-angiogenic. CVStC could be a promising biological source suitable for cell therapy applications in vascular diseases.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
