P. Bruschi, G.G. Vendramin, F. Bussotti, P. Grossoni (2000) - Morphological and molecular differentiation between Quercus petraea (Matt.) and Quercus pubescens (Willd.) in Northern and central Italy

Quercus petraea (Matt.) Liebl. and Q. pubescens Willd. are two closely related taxa. No speciÆc single marker, either morphological or molecular, has been identiÆed to distinguish the two species until now. In the present study morphoanatomical and molecular traits (simple sequence repeats à SSR loci) were investigated in an attempt to characterize and to discriminate between these two oak species. At the morphological level, the applied statistics (nested ANOVA and discriminant analysis) suggest that the micromorphological features are the most e€ective for this purpose. The wide range of macromorphological variation observed can confound the classiÆcation of many individuals into either Q. petraea and Q. pubescens . Analysis of microsatellite data indicates that most of the genetic variation is contained within, rather than between, species. The lack of molecular divergence could be attributed to either relatively rapid and recent di€erentiation or to extensive hybridization following secondary contact of the taxa. #