Mill-Hill-2 virus (MH2) proviral DNA was cloned from a transformed nonproducer [quail fibroblast] cell culture (MH2QB2) through insertion of randomly cut high MW cellular DNA in the lambdoid vector L47.1. Restriction analysis of a suitable recombinant phage by Southern DNA blotting and hybridization with different probes allowed characterization of the genetic organization of the provirus and identification of a novel MH2-specific sequence of at least 1.1 kilobase-pair. Such a sequence, v-mil, from Mill-Hill-2 virus, is not homologous to v-myc, the previously described oncogene of MH2, nor to avian leukemia virus-related sequences. Evidence is presented here that v-mil has a cellular counterpart (c-mil) phylogenetically conserved in birds and mammals, including man, and expressed as a single RNA species at least in some tissues. MH2 virus might thus be regarded, like avian erythroblastosis virus or E26, as another example of retroviruses having recombined with > 1 cellular gene.
MOLECULAR CLONING OF THE AVIAN ACUTE TRANSFORMING RETROVIRUS MH-2 REVEALS A NOVEL CELL DERIVED SEQUENCE V-MIL IN ADDITION TO THE MYC ONCOGENE
RIGHI M;
1983
Abstract
Mill-Hill-2 virus (MH2) proviral DNA was cloned from a transformed nonproducer [quail fibroblast] cell culture (MH2QB2) through insertion of randomly cut high MW cellular DNA in the lambdoid vector L47.1. Restriction analysis of a suitable recombinant phage by Southern DNA blotting and hybridization with different probes allowed characterization of the genetic organization of the provirus and identification of a novel MH2-specific sequence of at least 1.1 kilobase-pair. Such a sequence, v-mil, from Mill-Hill-2 virus, is not homologous to v-myc, the previously described oncogene of MH2, nor to avian leukemia virus-related sequences. Evidence is presented here that v-mil has a cellular counterpart (c-mil) phylogenetically conserved in birds and mammals, including man, and expressed as a single RNA species at least in some tissues. MH2 virus might thus be regarded, like avian erythroblastosis virus or E26, as another example of retroviruses having recombined with > 1 cellular gene.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
