Specimens of nematode belonging to Oscheius genis was isolated through the Galleria bait method from soil collected in a karst cave of Tuscany (Central Italy). Molecular and morphological analyses were performed. Total DNA was extracted from individual nematodes and the mitochondrial COI, the ITS containing region and the 18S rRNA gene were amplified and sequenced. BLAST search at the NCBI discriminate this new taxon, similar to other Oscheius. This species belongs to Dolichura group. Cuticle is finely annulated, stoma is short and cheilorhabdion is simple, not well cuticularized. Female body is almost straight upon fixation, the reproductive system is didelphic and tail is short, conoid with pointed tip. Males are rare and similar to female in general morphology except for smaller size. Male body is straight when heat-killed, testis is single, ventral reflexed. Thei show peloderan bursa , tail short rounded and spicules slender and small. Ingective juveniles are slender withelongate tail and have stoma morphology similar to adult. The nematodes were cultured in Petri dishes on several substrates: Nutrient Agar, Escherichia coli, Botritis cinerea, meat baby food, without satisfactory results. Only Petri dishes method with G. mallonella larvae produced IJs, suggesting the entomopathogenicityof this new taxon.
A new entomopathogenic Oscheius (Nematoda: Rhabditidae) from Italian cave
Fanelli E;De Luca F;Troccoli A;
2014
Abstract
Specimens of nematode belonging to Oscheius genis was isolated through the Galleria bait method from soil collected in a karst cave of Tuscany (Central Italy). Molecular and morphological analyses were performed. Total DNA was extracted from individual nematodes and the mitochondrial COI, the ITS containing region and the 18S rRNA gene were amplified and sequenced. BLAST search at the NCBI discriminate this new taxon, similar to other Oscheius. This species belongs to Dolichura group. Cuticle is finely annulated, stoma is short and cheilorhabdion is simple, not well cuticularized. Female body is almost straight upon fixation, the reproductive system is didelphic and tail is short, conoid with pointed tip. Males are rare and similar to female in general morphology except for smaller size. Male body is straight when heat-killed, testis is single, ventral reflexed. Thei show peloderan bursa , tail short rounded and spicules slender and small. Ingective juveniles are slender withelongate tail and have stoma morphology similar to adult. The nematodes were cultured in Petri dishes on several substrates: Nutrient Agar, Escherichia coli, Botritis cinerea, meat baby food, without satisfactory results. Only Petri dishes method with G. mallonella larvae produced IJs, suggesting the entomopathogenicityof this new taxon.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
