Aim: to investigate by molecular imaging the role of UCP3 protein in cardiac remodelling in a transgenic murine model of myocardial infarction. Methods: 20 control, and 15 wild type and 5 UCP3 knockout infartuated C57Bl/6J mice, 9-16 weeks aged, were analyzed with 18F-FDG PET/CT (eXplore VISTA GE, spatial resolution PET 1.4 mm FWHM, CT 200 ?m). Left ventricle mean uptake values (SUV, MBq/ml) have been measured with eXplore VISTA analysis tool in a representative axial slice; surface area and volume have been calculated in vivo with MunichHeart software from polar maps. Results: Left ventricle 18F-FDG mean uptake in healthy mice resulted 2.018±0.979 SUV/ 0.505±0.235 MBq/ml, whereas mean surface area and volume were 72.37 mm2 and 56.62 ?l respectively. 18F-FDG uptake in infartuated mice resulted 2.57±0.73 SUV/0.66±0.38 MBq/ml for wild type, 4.48±1.21 SUV/1.12±0.37 MBq/ml for UCP3 knockout mice (p 0.0031 and 0.002, Wilcoxon test, respectively). Mean values of surface area and volume in infartuated mice were 91.87 mm2 and 75 ?l, more than 20.5% (p 0.0034), and 17.7% (p 0.18, Wald test) of healthy mice, respectively, without significative changes over time ( p>0,05). Conclusions: UCP3 play a crucial role in mitochondrial energy metabolism and reactive oxygen species (ROS) generation. 18F-FDG PET in genetically engineered mice have emerged as important research tool to understand myocardial ischemic disease processes and to evaluate potential target of therapies.
18F-FDG-uptake by PET/CT in a Transgenic Murine Model of Myocardial Infarction.
S Gargiulo;M Gramanzini;
2013
Abstract
Aim: to investigate by molecular imaging the role of UCP3 protein in cardiac remodelling in a transgenic murine model of myocardial infarction. Methods: 20 control, and 15 wild type and 5 UCP3 knockout infartuated C57Bl/6J mice, 9-16 weeks aged, were analyzed with 18F-FDG PET/CT (eXplore VISTA GE, spatial resolution PET 1.4 mm FWHM, CT 200 ?m). Left ventricle mean uptake values (SUV, MBq/ml) have been measured with eXplore VISTA analysis tool in a representative axial slice; surface area and volume have been calculated in vivo with MunichHeart software from polar maps. Results: Left ventricle 18F-FDG mean uptake in healthy mice resulted 2.018±0.979 SUV/ 0.505±0.235 MBq/ml, whereas mean surface area and volume were 72.37 mm2 and 56.62 ?l respectively. 18F-FDG uptake in infartuated mice resulted 2.57±0.73 SUV/0.66±0.38 MBq/ml for wild type, 4.48±1.21 SUV/1.12±0.37 MBq/ml for UCP3 knockout mice (p 0.0031 and 0.002, Wilcoxon test, respectively). Mean values of surface area and volume in infartuated mice were 91.87 mm2 and 75 ?l, more than 20.5% (p 0.0034), and 17.7% (p 0.18, Wald test) of healthy mice, respectively, without significative changes over time ( p>0,05). Conclusions: UCP3 play a crucial role in mitochondrial energy metabolism and reactive oxygen species (ROS) generation. 18F-FDG PET in genetically engineered mice have emerged as important research tool to understand myocardial ischemic disease processes and to evaluate potential target of therapies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
