We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GRO alpha by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP(3)) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GRO alpha may play a neuromodulatory role on mouse cerebellum. (C) 1998 Elsevier Science B.V. All rights reserved.
CXC chemokines interleukin-8 (IL-8) and growth-related gene product alpha (GRO alpha) modulate Purkinje neuron activity in mouse cerebellum
Ciotti MT;Mercanti D;
1998
Abstract
We give here evidence that Purkinje neurons (PNs) of mouse cerebellar slices studied with patch clamp technique combined with laser confocal microscopy, respond to human IL-8 and GRO alpha by (i) a cytosolic Ca2+ transient compatible with inositol (1,4,5) trisphosphate (InsP(3)) formation; (ii) an enhancement of the neurotransmitter release; and (iii) an impairment of the long-term depression of synaptic strength (LTD). It was also found the expression of IL-8 receptor type 2 in PN and granule cells by immunofluorescence, immunoblotting and RT-PCR analysis. Considered together these findings suggest that IL-8 and GRO alpha may play a neuromodulatory role on mouse cerebellum. (C) 1998 Elsevier Science B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
