Cellular in vitro Testing: Methods and Protocols reports on a wide range of methods for the applications of 3D in vitro cell culture models, either for the purposes of in vitro testing or in the long-term development of forming tissues for therapeutic purposes. The scope and contents of the book have arisen from selected presentations given at the Annual Quasi-Vivo°R User Group Meetings, and the style of each chapter is based on a descriptive protocol style, such that readers will be able to reproduce individual methods in the laboratory step-by-step. The book starts with a review chapter, which gives an overview of methods for connected culture experiments using perfused flow chambers. Thereafter, there are nine chapters which cover key areas in human airway inhalation toxicity, quantifying xenobiotic metabolizing enzymes in hepatocytes, cardiac patches, risk assessment of nanoparticles, the development of nanosensor scaffolds, a paracrine human skin model for irritancy detection, drug testing using intestinal mucosa, a human bronchial model and the use of a porous scaffold for simple and routine 3D culture. We hope this book serves as a useful methods and protocols manual for laboratory scientists who need to develop the underpinning scientific basis and technical details for 3D in vitro cell cultures. We are personally indebted to all of the international experts who have kindly contributed chapters and taken great care and time in preparing their contributions for this book.
In vitro Risk Assessment of Nanoparticles
Ucciferri Nadia;
2015
Abstract
Cellular in vitro Testing: Methods and Protocols reports on a wide range of methods for the applications of 3D in vitro cell culture models, either for the purposes of in vitro testing or in the long-term development of forming tissues for therapeutic purposes. The scope and contents of the book have arisen from selected presentations given at the Annual Quasi-Vivo°R User Group Meetings, and the style of each chapter is based on a descriptive protocol style, such that readers will be able to reproduce individual methods in the laboratory step-by-step. The book starts with a review chapter, which gives an overview of methods for connected culture experiments using perfused flow chambers. Thereafter, there are nine chapters which cover key areas in human airway inhalation toxicity, quantifying xenobiotic metabolizing enzymes in hepatocytes, cardiac patches, risk assessment of nanoparticles, the development of nanosensor scaffolds, a paracrine human skin model for irritancy detection, drug testing using intestinal mucosa, a human bronchial model and the use of a porous scaffold for simple and routine 3D culture. We hope this book serves as a useful methods and protocols manual for laboratory scientists who need to develop the underpinning scientific basis and technical details for 3D in vitro cell cultures. We are personally indebted to all of the international experts who have kindly contributed chapters and taken great care and time in preparing their contributions for this book.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.