Urine metabolomic profiles by 1H NMR spettroscopy and MVA of piglets fed with mycotoxin-contaminated boluses

Deoxynivalenol (DON), aflatoxin B1 (AFB1), fumonisin B1 (FB1), zearalenone (ZEA) and ochratoxin A (OTA) are toxicologically relevant mycotoxins frequently occurring in cereals and cereal-based feeds and recently quantified in vivo in piglets urines in dose dependent manner [1]. The possible metabolic effects due to mycotoxins exposure were evaluated by 1H-NMR spectroscopy and MVA (Multivariate Analysis) of exposed piglets urine. Four piglet groups (four animals per group) received feed boluses containing DON, AFB1, FB1, ZEA, and OTA at four increasing doses (one bolus per piglet). The 24 h post dose urines were collected, analysed and results were compared with the controls (urines from the same piglets sampled the day before administration). Interestingly, the urine from piglets fed with high contaminated boluses showed higher content of p-cresol glucoronide, phenyl acetyl glycine and creatinine and lower level of TMAO with respect to the control. [1]. Gambacorta L, Solfrizzo M, Visconti A, Powers S, Cossalter AM, Pinton P, Oswald IP, Validation study on urinary biomarkers of exposure for aflatoxin B1, ochratoxin A, fumonisin B1, deoxynivalenol and zearalenone in piglets. World Mycotoxin Journal. 2013 6(3): 299 - 308.