As previously reported, a single administration of testosterone propionate (TP) in ex-hypoxic polycythemic mice induced an 18-24 h amplification of the erythroid burst-forming unit (BFU-E) pool and a 60-h expansion of the erythroid colony-forming unit (CFU-E) compartment. In this study both phenomena were temporally associated with an increase of the in vitro 3H-TdR sensitivity of these compartments, thus indicating an elevation of their proliferative rate. No significant modification of the DNA synthesis index and the pool size of BFU-E and CFU-E were observed at, respectively, 60 or 18 h. At either time interval, 3H-TdR sensitivity and compartment size were not modified at the level of the myeloid-macrophage colony-forming unit (CFU-C). The early and late expansion of, respectively, BFU-E and CFU-E number after TP injection is apparently at least partially mediated by enhancement of the proliferative rate within the respective compartments. Mechanisms underlying TP action on BFU-E and CFU-E pools were discussed in the light of present and previous observations.
TESTOSTERONE ACTION ON ERYTHROPOIETIC BURST FORMING UNITS AND ERYTHROID COLONY FORMING UNITS CORRELATION BETWEEN ENHANCEMENT OF PROLIFERATIVE RATE AND AMPLIFICATION OF POOL SIZE
MAGLI M C;
1979
Abstract
As previously reported, a single administration of testosterone propionate (TP) in ex-hypoxic polycythemic mice induced an 18-24 h amplification of the erythroid burst-forming unit (BFU-E) pool and a 60-h expansion of the erythroid colony-forming unit (CFU-E) compartment. In this study both phenomena were temporally associated with an increase of the in vitro 3H-TdR sensitivity of these compartments, thus indicating an elevation of their proliferative rate. No significant modification of the DNA synthesis index and the pool size of BFU-E and CFU-E were observed at, respectively, 60 or 18 h. At either time interval, 3H-TdR sensitivity and compartment size were not modified at the level of the myeloid-macrophage colony-forming unit (CFU-C). The early and late expansion of, respectively, BFU-E and CFU-E number after TP injection is apparently at least partially mediated by enhancement of the proliferative rate within the respective compartments. Mechanisms underlying TP action on BFU-E and CFU-E pools were discussed in the light of present and previous observations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.