We report the non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a microspherical whispering gallery mode resonator (WGMR). The immunoreagents have been immobilized onto the surface of the WGMR sensor after being activated with an epoxy silane and an orienting layer. The developed immunosensor presents great potential as a robust sensing device for fast and early detection of immunoreactions. We also tested the potential of microbubbles as nonlinear enhancement platform. The dyes used in these studies are tetramethyl rhodamine isothiocyanate and Rhodamine 6G. All measurements were performed in a modified confocal microscope.
Non-linear fluorescence excitation of Rhodamine 6G and TRITC labeled IgG in whispering gallery mode microresonators
Farnesi Daniele;Farnesi Daniele;Berneschi Simone;
2015
Abstract
We report the non linear fluorescence real-time detection of labeled IgG covalently bonded to the surface of a microspherical whispering gallery mode resonator (WGMR). The immunoreagents have been immobilized onto the surface of the WGMR sensor after being activated with an epoxy silane and an orienting layer. The developed immunosensor presents great potential as a robust sensing device for fast and early detection of immunoreactions. We also tested the potential of microbubbles as nonlinear enhancement platform. The dyes used in these studies are tetramethyl rhodamine isothiocyanate and Rhodamine 6G. All measurements were performed in a modified confocal microscope.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
