Integrated andrological evaluation (IAE) is a practice to identify Satisfactory (S), Questionable (Q) and Unsatisfactory (US) males. IAE procedure mainly involves classical and modern methods. The routine evaluation system contains physical and reproductive examination, while the innovative approach is more based on ultrasound detection of testicular and accessory glands, scrotal surface thermography, GnRH challenge test, CASA semen analysis (Computer Assisted Semen Analyzer), fluorescent staining, seminal plasma biochemistry, testicular fine needle aspiration cytology (TFNAC). The aim of this trial was to conduct a pilot study with an IAE based evaluation system (except of the TFNAC) in four healthy adult (4/6 years) Angora goat in Kazan-Turkey. Physical traits such as age, BSC, hereditable defects on: eyes, mouth, legs/feet, prepuce, penis, scrotum and its components were recorded and scrotal circumference, testicular ultrasound (ESAOTE MyLab5, Genoa, Italy with convex probe 2.2 - 6.6 MHz) and scrotal thermography (Flir, E60 during GnRH challenge test with 8.4 ?g Busereline IV) were performed. Semen parameters such as color, volume, concentration, sperm motility (SCA Microptics), viability and morphology (Eosin-Nigrosin stain), acrosome integrity (FITC-PNA) were measured in fresh and frozen-thawed semen samples. Correlation indices and mathematical tendencies were calculated using Sigma Stat Software 2.05 and Microsoft Excel version 14.4.9. Three males were evaluated as Q, and one as US because of the presence of feet and mouth defects. One buck has not been evaluated by reasons of higher delta Testosteronemia during GnRH Challenge Test and echotexture testicular classification (Lower Mineralization Index). All mature bucks showed similar scrotal thermal pattern. Seminal plasma mean values of cholesterol, glucose, LDH, triglycerides, total protein, GGT and magnesium were 30.5 mg/dl, 77.8 mg/dl, 470.1 u/L, 8.8 mg/dl, 82.5 g/l, 46.8 u/L and 2.03 mg/dl, respectively. Bucks with higher testicular functionality, according to the physical examination, had the best freezability (Delta Viability and Intact Acrosome) and higher levels of cholesterol (34.5 mg/dl) glucose (87.4 mg/dl), LDH (585.4 u/L), triglycerides (11.15 mg/dl), total protein (89.0 g/l), GGT (53.4 u/L) as well as the lower levels of magnesium (1.88 mg/dl) in the seminal plasma. A correlation between testicular functionality and frozenthawed semen parameters was also confirmed by sperm kinetic parameters, viability and morphology results. Application of IAE in Angora goat may indicate the buck selection for specific purposes such as breeding, cryopreservation or exclusion from any application.
Integreated andrological evaluation in Angora goat
F Turri;F Pizzi
2015
Abstract
Integrated andrological evaluation (IAE) is a practice to identify Satisfactory (S), Questionable (Q) and Unsatisfactory (US) males. IAE procedure mainly involves classical and modern methods. The routine evaluation system contains physical and reproductive examination, while the innovative approach is more based on ultrasound detection of testicular and accessory glands, scrotal surface thermography, GnRH challenge test, CASA semen analysis (Computer Assisted Semen Analyzer), fluorescent staining, seminal plasma biochemistry, testicular fine needle aspiration cytology (TFNAC). The aim of this trial was to conduct a pilot study with an IAE based evaluation system (except of the TFNAC) in four healthy adult (4/6 years) Angora goat in Kazan-Turkey. Physical traits such as age, BSC, hereditable defects on: eyes, mouth, legs/feet, prepuce, penis, scrotum and its components were recorded and scrotal circumference, testicular ultrasound (ESAOTE MyLab5, Genoa, Italy with convex probe 2.2 - 6.6 MHz) and scrotal thermography (Flir, E60 during GnRH challenge test with 8.4 ?g Busereline IV) were performed. Semen parameters such as color, volume, concentration, sperm motility (SCA Microptics), viability and morphology (Eosin-Nigrosin stain), acrosome integrity (FITC-PNA) were measured in fresh and frozen-thawed semen samples. Correlation indices and mathematical tendencies were calculated using Sigma Stat Software 2.05 and Microsoft Excel version 14.4.9. Three males were evaluated as Q, and one as US because of the presence of feet and mouth defects. One buck has not been evaluated by reasons of higher delta Testosteronemia during GnRH Challenge Test and echotexture testicular classification (Lower Mineralization Index). All mature bucks showed similar scrotal thermal pattern. Seminal plasma mean values of cholesterol, glucose, LDH, triglycerides, total protein, GGT and magnesium were 30.5 mg/dl, 77.8 mg/dl, 470.1 u/L, 8.8 mg/dl, 82.5 g/l, 46.8 u/L and 2.03 mg/dl, respectively. Bucks with higher testicular functionality, according to the physical examination, had the best freezability (Delta Viability and Intact Acrosome) and higher levels of cholesterol (34.5 mg/dl) glucose (87.4 mg/dl), LDH (585.4 u/L), triglycerides (11.15 mg/dl), total protein (89.0 g/l), GGT (53.4 u/L) as well as the lower levels of magnesium (1.88 mg/dl) in the seminal plasma. A correlation between testicular functionality and frozenthawed semen parameters was also confirmed by sperm kinetic parameters, viability and morphology results. Application of IAE in Angora goat may indicate the buck selection for specific purposes such as breeding, cryopreservation or exclusion from any application.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
