Etiolation of vegetables is a complex phenomenon implying qualitative and quantitative protein changes. The 2-DE protein profile of green and etiolated fleshy stalk samples has shown great differences in the 4-7 pH and 10 to 250 kDa mass range. Currently, a shortage ofCynaraDNA or protein sequences has required the use ofde novosequencing and BLAST similarity searches for protein identification. Highlighted herein is an application ofproteomics to the identification of proteins, which in a great part matched those from theViridiplantaeorder. Quantitative evaluation, statistical analyses, and MALDI-TOF MS characterization of the resolved spots in green and etiolated samples ofC. cardunculusenabled us to identify 46 out of 60 spots, containing 21 spots included conservedViridiplantae domains.The etiolation process is discussed in light of the identification of some proteins involved in specific biochemical and metabolic pathways.
The Proteomic Changes in Cynara Cardunculus L. var.altilis DC Following the Etiolation Phenomena UsingDe Novo Sequence Analysis
2010
Abstract
Etiolation of vegetables is a complex phenomenon implying qualitative and quantitative protein changes. The 2-DE protein profile of green and etiolated fleshy stalk samples has shown great differences in the 4-7 pH and 10 to 250 kDa mass range. Currently, a shortage ofCynaraDNA or protein sequences has required the use ofde novosequencing and BLAST similarity searches for protein identification. Highlighted herein is an application ofproteomics to the identification of proteins, which in a great part matched those from theViridiplantaeorder. Quantitative evaluation, statistical analyses, and MALDI-TOF MS characterization of the resolved spots in green and etiolated samples ofC. cardunculusenabled us to identify 46 out of 60 spots, containing 21 spots included conservedViridiplantae domains.The etiolation process is discussed in light of the identification of some proteins involved in specific biochemical and metabolic pathways.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
