In this chapter, we review the theoretical and experimental foundations underling a quantitative approach to Ca2+ imaging, discuss equilibrium conditions and their violations and present a computational framework that can be used to estimate the spatial and temporal dynamics of Ca2+ signals based of fluorescence measurements with Ca2+ indicators.

Ca2+ Imaging: Principles of Analysis and Enhancement

Mammano Fabio;Bortolozzi Mario
2010

Abstract

In this chapter, we review the theoretical and experimental foundations underling a quantitative approach to Ca2+ imaging, discuss equilibrium conditions and their violations and present a computational framework that can be used to estimate the spatial and temporal dynamics of Ca2+ signals based of fluorescence measurements with Ca2+ indicators.
2010
Istituto di Neuroscienze - IN -
Istituto di Biologia Cellulare e Neurobiologia - IBCN - Sede Monterotondo Scalo
Istituto di Biochimica e Biologia Cellulare - IBBC
978-1-60761-475-3
Buffers
Law of mass action
Binding reactions
Fluorescent indicators
Optical measurement of Ca2+ concentration
Single wavelength and ratiometric imaging
Chemical equilibrium
Non-equilibrium conditions
Diffusion
Ion fluxes
Differential equations
Monte Carlo methods
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/309255
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 10
social impact