We have sought to investigate the responses of Nerve Growth Factor (NGF) receptors TrkA and P75NTR at the plasma membrane of living neuronal cells by single-molecule imaging and tracking. To this purpose we exploit the acyl carrier peptide and some of its shortened versions (A1 and S6 tags, labeled selectively by two different PPTases) to tag human p75NTR and TrkA. These tags were covalently conjugated to the biotin- or fluorophore-substituted arm of a coenzyme A (CoA) substrate.
Ligand Fingerprinting in the Membrane Dynamics of Single TrkA and P75NTR Neurotrophin Receptors
Stefano Luin;Fabio Beltram;
2015
Abstract
We have sought to investigate the responses of Nerve Growth Factor (NGF) receptors TrkA and P75NTR at the plasma membrane of living neuronal cells by single-molecule imaging and tracking. To this purpose we exploit the acyl carrier peptide and some of its shortened versions (A1 and S6 tags, labeled selectively by two different PPTases) to tag human p75NTR and TrkA. These tags were covalently conjugated to the biotin- or fluorophore-substituted arm of a coenzyme A (CoA) substrate.File in questo prodotto:
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