he present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8T. This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures (4 and 15 °C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC-FID (gas chromatography- flame ionization detection) hydrocarbon analysis. Results from DNA extraction and DAPI (4?,6-diamidino-2-phenylindole) staining showed a linear increase during the first 18 days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC-FID results revealed a substan- tial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15 °C rather than 4 °C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4 °C.
Biodegradation potentiality of psychrophilic bacterial strain Oleispira antarctica RB-8T
2016
Abstract
he present study is focused on assessing the growth and hydrocarbon-degrading capability of the psychrophilic strain Oleispira antarctica RB-8T. This study considered six hydrocarbon mixtures that were tested for 22 days at two different cultivation temperatures (4 and 15 °C). During the incubation period, six sub-aliquots of each culture at different times were processed for total bacterial abundance and GC-FID (gas chromatography- flame ionization detection) hydrocarbon analysis. Results from DNA extraction and DAPI (4?,6-diamidino-2-phenylindole) staining showed a linear increase during the first 18 days of the experiment in almost all the substrates used; both techniques showed a good match, but the difference in values obtained was approximately one order of magnitude. GC-FID results revealed a substan- tial hydrocarbon degradation rate in almost all hydrocarbon sources and in particular at 15 °C rather than 4 °C (for commercial oil engine, oily waste, fuel jet, and crude oil). A more efficient degradation was observed in cultures grown with diesel and bilge water at 4 °C.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
