A. colubrina var. cebil (Vell.) Brenan represents a trace in memory of the forests that decreased over the years and this is the reason why it's interesting to study and to preserve it. This is an important tree for cultural, economic, and medicinal uses in South America. In order to implement a policy of conservation of A. colubrina, we have characterized the genetic diversity of 4 populations. We collected seeds from 4 different sites (B San Bernardo, C El Cebilar, M Metán and G El Gallinato) in Salta Province. Then, we compared the intergenic transcribed sequences (ITS) of ribosomal DNA (rDNA), a known molecular marker. Our previous results (1), obtained through morphological and genetic analysis of only 4 individuals (one per zone), have showed that B and M individuals were more similar each other, as well as G and C. Here, a largest number of individuals (29) were characterized, and their phylogenetic relationships analysed. The results comfirmed the previously found similarities, indicating that the genetic characteristics of A. colubrina populations follow a geographic distribution, probably related to the environmental climate. Besides, we found that populations of A. colubrina from the Salta province showed high genetic distance and different haplotypes in ITS-rDNA, that could be a consequence of different colonization events. This finding is expected in forest trees due to high population sizes, longevity of individuals, and high levels of gene flow between populations. As tree have a crucial role in the social, ecological and economic life, covering the 30% of Earth, the study of plant diversity may help conservation of forests.
Phylogenetics of Anadenanthera colubrina (var. cebil) tree from Salta (Northwest Argentina)
Mirella Ciaccio;Roberta Russo;
2015
Abstract
A. colubrina var. cebil (Vell.) Brenan represents a trace in memory of the forests that decreased over the years and this is the reason why it's interesting to study and to preserve it. This is an important tree for cultural, economic, and medicinal uses in South America. In order to implement a policy of conservation of A. colubrina, we have characterized the genetic diversity of 4 populations. We collected seeds from 4 different sites (B San Bernardo, C El Cebilar, M Metán and G El Gallinato) in Salta Province. Then, we compared the intergenic transcribed sequences (ITS) of ribosomal DNA (rDNA), a known molecular marker. Our previous results (1), obtained through morphological and genetic analysis of only 4 individuals (one per zone), have showed that B and M individuals were more similar each other, as well as G and C. Here, a largest number of individuals (29) were characterized, and their phylogenetic relationships analysed. The results comfirmed the previously found similarities, indicating that the genetic characteristics of A. colubrina populations follow a geographic distribution, probably related to the environmental climate. Besides, we found that populations of A. colubrina from the Salta province showed high genetic distance and different haplotypes in ITS-rDNA, that could be a consequence of different colonization events. This finding is expected in forest trees due to high population sizes, longevity of individuals, and high levels of gene flow between populations. As tree have a crucial role in the social, ecological and economic life, covering the 30% of Earth, the study of plant diversity may help conservation of forests.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
