RESEARCH DESIGN AND METHODS-The content of fatty acids in phospholipids of INS-1E beta-cells was determined by lipidomics analysis. High-performance liquid chromatography was used to identify peroxidation products in beta-cell cultures. Static and dynamic glucose-stimulated insulin secretion (GSIS) assays were performed on isolated rat islets and/or INS-1E cells. The function of peroxisome proliferator-activated receptor-delta (PPAR-delta) in regulating insulin secretion was investigated using pharmacological agents and gene expression manipulations.
OBJECTIVE-Previous studies show that polyunsaturated fatty acids (PUFAs) increase the insulin secretory capacity of pancreatic beta-cells. We aimed at identifying PUFA-derived mediators and their cellular targets that are involved in the amplification of insulin release from beta-cells preexposed to high glucose levels.
Role of Lipid Peroxidation and PPAR-delta in Amplifying Glucose-Stimulated Insulin Secretion
Chatgilialoglu Chryssostomos;Ferreri Carla;
2011
Abstract
OBJECTIVE-Previous studies show that polyunsaturated fatty acids (PUFAs) increase the insulin secretory capacity of pancreatic beta-cells. We aimed at identifying PUFA-derived mediators and their cellular targets that are involved in the amplification of insulin release from beta-cells preexposed to high glucose levels.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
