New polymeric sorbent for the solid-phase extraction of indole-3-acetic acid from plants followed by liquid chromatography - Fluorescence detector

This study reports on the development of a newly synthesized polymer sorbent for the analysis of indole-3-acetic acid (IAA), which is the most important auxin in plants, from complex raw matrices, such as plant extracts. IAA was determined by high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FD). The polymer developed was able to bind about 94% of IAA with a reproducible manner. The release of IAA in methanol was more than 70% for both of them. Non-specific hydrophobic interactions are, likely, the dominant driving force of the binding between IAA and the polymer.

Plant hormones play a crucial role in controlling plant growth and development. These groups of naturally occurring substances trigger physiological processes at very low concentrations, which require sensitive techniques for their quantitation.