In this study, a sandwich ELISA was developed to address both rapidity and specificity in order to detect the p60 protein secreted by Listeria monocytogenes, a harmful foodborne bacterial pathogen. By sequentially combining the use of a monoclonal antibody against a L. monocytogenes specific 11-amino acids peptide sequence on the protein p60 and polyclonal antibodies against the whole p60 protein, 103 CFU/ml of L. monocytogenes were specifically detected without cross reaction with Listeria innocua, Escherichia coli, Salmonella Typhimurium and S. aureus. The detection was preceded by an incubation period of 18 h with minimal experimental manipulations. Among the sample preparation procedures tested, samples directly from L. monocytogenes bacterial culture allowed better detection as compared to cell-free supernatant samples. This sandwich ELISA is an experimental design that could be easily adapted for use in food processing industries for routine monitoring of washing and sanitizing procedures.
Development of a highly specific sandwich ELISA for the detection of Listeria monocytogenes, an important foodborne pathogen
Sabato D'Auria;
2014
Abstract
In this study, a sandwich ELISA was developed to address both rapidity and specificity in order to detect the p60 protein secreted by Listeria monocytogenes, a harmful foodborne bacterial pathogen. By sequentially combining the use of a monoclonal antibody against a L. monocytogenes specific 11-amino acids peptide sequence on the protein p60 and polyclonal antibodies against the whole p60 protein, 103 CFU/ml of L. monocytogenes were specifically detected without cross reaction with Listeria innocua, Escherichia coli, Salmonella Typhimurium and S. aureus. The detection was preceded by an incubation period of 18 h with minimal experimental manipulations. Among the sample preparation procedures tested, samples directly from L. monocytogenes bacterial culture allowed better detection as compared to cell-free supernatant samples. This sandwich ELISA is an experimental design that could be easily adapted for use in food processing industries for routine monitoring of washing and sanitizing procedures.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
