Quenching a [human] Hb solution partially saturated with CO into a hydro-organic solvent containing ferricyanide will produce under suitable conditions a population of partially oxidized and CO-bound Hb molecules. Since each Fe3+ heme carries 1 extra charge, it should be possible, in theory, to resolve the spectrum of intermediate compounds between Hb and CO, which was originally present in solution. The following are discussed: the development of a simple and rapid method to quench aqueous Hb solutions into a hydro-organic solvent at subzero temperatures; the determination of suitable experimental conditions to isolate valence hybrids between HbCO and methemoglobin by isoelectric focusing at temperatures as low as -25.degree. C; and the identification and isolation of all valence hybrids of different charge between HbCO and methemoglobin.
ISOLATION OF INTERMEDIATE VALENCE HYBRIDS BETWEEN FERROUS AND MET HEMO GLOBIN AT SUBZERO TEMPERATURES
BENAZZI L;
1981
Abstract
Quenching a [human] Hb solution partially saturated with CO into a hydro-organic solvent containing ferricyanide will produce under suitable conditions a population of partially oxidized and CO-bound Hb molecules. Since each Fe3+ heme carries 1 extra charge, it should be possible, in theory, to resolve the spectrum of intermediate compounds between Hb and CO, which was originally present in solution. The following are discussed: the development of a simple and rapid method to quench aqueous Hb solutions into a hydro-organic solvent at subzero temperatures; the determination of suitable experimental conditions to isolate valence hybrids between HbCO and methemoglobin by isoelectric focusing at temperatures as low as -25.degree. C; and the identification and isolation of all valence hybrids of different charge between HbCO and methemoglobin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
