The adsorption/ release behavior of oligodeoxynucleotides (ODNs) on new PEGylated coreshell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.
Core-shell nanospheres for oligonucleotide delivery. V: Adsorption/release behaviour of stealth nanospheres
L Tondelli;
2003
Abstract
The adsorption/ release behavior of oligodeoxynucleotides (ODNs) on new PEGylated coreshell polymethylmethacrylate nanospheres is described. The outer shell consists of alkyl chains containing quaternary ammonium groups and of poly(ethylene glycol) chains, both covalently bound to the inner core. Ion pair formation between negatively charged ODN phosphate groups and positively charged groups on the nanosphere surface is the main interaction mechanism. No cellular toxicity in HL60 cells is observed at nanosphere concentrations required for biologically active ODN delivery. These results indicate that these novel cationic polymeric nanoparticles are safe and represent promising vectors for oligonucleotide delivery.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
