An hydrolytic technique that stains constitutive heterochromatin of wheat is described. This technique, that is applicable to chromosomes squashed after hot HCl hydrolysis, produces a banding pattern similar to that produced by Barium-Saline-Giemsa, and is less time consuming and more reliable. The karyotype of durum wheat is described after the use of the double-ditelosomic lines of the cv. Langdon to inequivocally identify single chromosomes.
DIFFERENTIAL STAINING OF WHEAT CHROMOSOMES WITH AN HYDROLYTIC TECHNIQUE
PIGNONE D;CIFARELLI S
1989
Abstract
An hydrolytic technique that stains constitutive heterochromatin of wheat is described. This technique, that is applicable to chromosomes squashed after hot HCl hydrolysis, produces a banding pattern similar to that produced by Barium-Saline-Giemsa, and is less time consuming and more reliable. The karyotype of durum wheat is described after the use of the double-ditelosomic lines of the cv. Langdon to inequivocally identify single chromosomes.File in questo prodotto:
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