The activities hitherto carried out within the PhD research project are the following: 1) optimization of the amperometric bioprobe based on Saccharomyces cerevisiae cells for studying the metabolic/mitochondrial effects of four potential contaminants of milk, and 2) exploitation of Chlamydomonas reinhardtii and genetically engineered Escherichia coli ATCC 11303 as biomediators for the optical bioprobe for fluoroquinolones detection through chlorophyll a transient fluorescence measurements and optical (fluorescence or luminescence) measurements respectively.
Le attività svolte sinora nell'ambito del progetto di ricerca di dottorato sono le seguenti: 1) ottimizzazione della biosonda amperometrica a cellule di Saccharomyces cerevisiae per lo studio degli effetti metabolici/mitocondriali di quattro potenziali contaminanti del latte e 2) utilizzo di Chlamydomonas reinhardtii ed Escherichia coli ATCC 11303 geneticamente ingegnerizzato come biomediatori per la biosonda ottica per il rilevamento di fluorochinoloni rispettivamente attraverso misure di fluorescenza transiente della clorofilla a e misure ottiche (fluorescenza o luminescenza).
Engineered Bioprobes for the Identification of Chemical-Toxicological Hazards in Foods
Gerardo Grasso
2013
Abstract
The activities hitherto carried out within the PhD research project are the following: 1) optimization of the amperometric bioprobe based on Saccharomyces cerevisiae cells for studying the metabolic/mitochondrial effects of four potential contaminants of milk, and 2) exploitation of Chlamydomonas reinhardtii and genetically engineered Escherichia coli ATCC 11303 as biomediators for the optical bioprobe for fluoroquinolones detection through chlorophyll a transient fluorescence measurements and optical (fluorescence or luminescence) measurements respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
