The success of the grape and wine-related agribusiness depends on yield and quality of the grapes produced, which is heavily influenced by genetic diversity (cultivars and clones) and environment (terroir). Among the important Italian varieties, 'Nebbiolo' is one of the best examples of this connection. The behavior of three 'Nebbiolo' clones (CVT71, CVT185, CVT423) grafted on the same rootstock (Kober 5BB) and grown in three different experimental vineyards in the typical growing areas of Piedmont (Italy), was analyzed in relation to the different soil and environmental conditions. In two years (2013 and 2014) the agronomical and biochemical performances of those vines were monitored from winter pruning to harvest, recording vigor, yield, juice composition and anthocyanin profile. RNA-Seq analyses were performed on berries collected in three different developmental stages during the vegetative season: berry pea size stage, veraison and at harvest (24 °Brix). RNA-seq data were analyzed through a data mining process with the aim of investigating the relationships between time course of gene expression profiles and environmental variables, and of quantifying how much these variables affect the gene expression. The most important changes in the berry transcriptome are related to the different developmental stages. However, also vineyard and genotype induce significant modulation of several genes. For example, in a vineyard with a less favorable exposure we observed the activation of many genes related to stress responses. Another example is given by the clone CVT423, which showed different agronomic and metabolic performances with respect to the other two clones as well as different modulation of several genes. This research was funded by Fondazione Cassa di Risparmio di Cuneo, Project "Nebbiolo Genomics".
Transcriptomic changes in 'Nebbiolo' clones cultivated in different growing areas of Piedmont (Italy).
Gambino G;Boccacci P;Cuozzo D;Chitarra W;Perrone I;Mannini F;Gribaudo I
2016
Abstract
The success of the grape and wine-related agribusiness depends on yield and quality of the grapes produced, which is heavily influenced by genetic diversity (cultivars and clones) and environment (terroir). Among the important Italian varieties, 'Nebbiolo' is one of the best examples of this connection. The behavior of three 'Nebbiolo' clones (CVT71, CVT185, CVT423) grafted on the same rootstock (Kober 5BB) and grown in three different experimental vineyards in the typical growing areas of Piedmont (Italy), was analyzed in relation to the different soil and environmental conditions. In two years (2013 and 2014) the agronomical and biochemical performances of those vines were monitored from winter pruning to harvest, recording vigor, yield, juice composition and anthocyanin profile. RNA-Seq analyses were performed on berries collected in three different developmental stages during the vegetative season: berry pea size stage, veraison and at harvest (24 °Brix). RNA-seq data were analyzed through a data mining process with the aim of investigating the relationships between time course of gene expression profiles and environmental variables, and of quantifying how much these variables affect the gene expression. The most important changes in the berry transcriptome are related to the different developmental stages. However, also vineyard and genotype induce significant modulation of several genes. For example, in a vineyard with a less favorable exposure we observed the activation of many genes related to stress responses. Another example is given by the clone CVT423, which showed different agronomic and metabolic performances with respect to the other two clones as well as different modulation of several genes. This research was funded by Fondazione Cassa di Risparmio di Cuneo, Project "Nebbiolo Genomics".I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
