Climate change phenomenon, soil qualities and rootstocks are among the main factors that influence grape development as well as fruit and wine composition. The relationships between scion and biotic/abiotic factors are rootstock-mediated. Studies investigating the molecular network of rootstock and scion interactions are still scarce and the selection of the appropriate rootstock is and will be one of the key factor for the development of a more sustainable viticulture. In this work, the rootstock/scion interactions were studied using a whole-transcriptome approach on leaves of wine red variety 'Gaglioppo', grafted onto thirteen different rootstocks growing in the same vineyard in adjacent rows. The experiments were performed during the summer season 2014 in a vineyard located in Calabria Region (South-east Italy) that falls to Mediterranean climate characterized by warm and dry summers. Leaf transcriptome of 'Gaglioppo' grafted on five selected rootstocks showed high variability in gene expression. In particular, significant modulation of transcripts linked to primary (e.g. carbohydrate and transport) and secondary metabolism (e.g. phenylpropanoids pathways and response to stress) was observed. Interestingly, genes involved in the priming defense responses (e.g stilbenes and defense genes) were strongly activated only in some graft combinations. These results were further confirmed by the quantification of stilbene contents by HPLC-MS/MS. The adopted approach allowed to point out an interesting result among the others: the leaves of 'Gaglioppo' grafted on 1103 Paulsen showed the lowest levels of stilbene synthase transcripts and stilbene compound and revealed interestingly the greater sensitivity to downy mildew in in vitro assay. This study carrying out an extensive analysis of the rootstock effects on scion leaves open the possibility to unravel this complex interaction in a useful way considering the ongoing climate changes.
Rootstock x scion interactions in grapevine: transcript and metabolite modulation.
Perrone I;Chitarra W;Boccacci P;Santini D;Mannini F;Gambino G
2016
Abstract
Climate change phenomenon, soil qualities and rootstocks are among the main factors that influence grape development as well as fruit and wine composition. The relationships between scion and biotic/abiotic factors are rootstock-mediated. Studies investigating the molecular network of rootstock and scion interactions are still scarce and the selection of the appropriate rootstock is and will be one of the key factor for the development of a more sustainable viticulture. In this work, the rootstock/scion interactions were studied using a whole-transcriptome approach on leaves of wine red variety 'Gaglioppo', grafted onto thirteen different rootstocks growing in the same vineyard in adjacent rows. The experiments were performed during the summer season 2014 in a vineyard located in Calabria Region (South-east Italy) that falls to Mediterranean climate characterized by warm and dry summers. Leaf transcriptome of 'Gaglioppo' grafted on five selected rootstocks showed high variability in gene expression. In particular, significant modulation of transcripts linked to primary (e.g. carbohydrate and transport) and secondary metabolism (e.g. phenylpropanoids pathways and response to stress) was observed. Interestingly, genes involved in the priming defense responses (e.g stilbenes and defense genes) were strongly activated only in some graft combinations. These results were further confirmed by the quantification of stilbene contents by HPLC-MS/MS. The adopted approach allowed to point out an interesting result among the others: the leaves of 'Gaglioppo' grafted on 1103 Paulsen showed the lowest levels of stilbene synthase transcripts and stilbene compound and revealed interestingly the greater sensitivity to downy mildew in in vitro assay. This study carrying out an extensive analysis of the rootstock effects on scion leaves open the possibility to unravel this complex interaction in a useful way considering the ongoing climate changes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
