Introduction:The plant LTP family consists of ubiquitous proteinstypically involved in defense mechanisms against the attack of bacte-ria, fungi and viruses. They constitute an important cause of allergicsensitization in the Mediterranean area. A new nanobead-based IVDtest for specific IgE detection using 122 molecular allergens and 122allergenic extracts, all coupled to chemically activated nano-particles.Eight LTP purified from kiwi, hazelnut, pomegranate, peach, walnut,corn, tomato and wheat are immobilized together with 25 extractsfrom plant-derived foods bearing, along with other allergens, theirLTP.Objectives:To describe the LTP sensitization in a population of1751 patients by using molecules and extracts.Results:Out of the 1751 routinely tested patients 208 (11.88%)turned out to be sensitized to at least one LTP. The sensitizationprevalence of each specific LTP within the 208 patients wasdetected as follows: Pru p 3 94%, Jug r 3 49%, Cor a 8 30%, Zea m14 30%, Pun g 1 23%, Act d 10 21%, Tri a 7k-LTP 3%, Sola l 6 2%.Pru p 3 reaches 100% prevalence in pediatric patients between 0 to10 years old population. The 6% Pru p 3 negative subjects turnedout to be mono-sensitized to one of the other tested LTP. The avail-ability of extracts from many other vegetables, fruits and seedsallowed the definition of the sensitization to a broader number ofrelevant LTP. An IgE reactivity was detected to allergenic sourceswhose content in LTP is still unknown. A different IgE reactivity hasbeen recorded for the 9 k-LTP and the 7 k-LTP. Patients recognizingthe latter group have a broader recognition pattern and higher IgElevels.Conclusions:The specific advantage of a MULTIPLEX isle testrelies on the chance of testing patients to a broader panel of LTP aswell as to a large number of extracts, complementing the results onthe single allergenic molecules.The availability of the MULTIPLEX test of allergens belonging to theBet v 1-like proteins, Profilins, CCD markers and Anti-Microbial Pep-tide groups allows to greatly improve the patient sensitization profil-ing. The use of extracts to consumed food or newly introduced onesincreases our knowledge on allergens.
Multiplex IgE diagnostic test: the most comprehensive view on IgE sensitization to vegetables, fruits and seeds due to lipid transfer proteins
Tuppo L;Tamburrini M;Ciardiello M A;
2017
Abstract
Introduction:The plant LTP family consists of ubiquitous proteinstypically involved in defense mechanisms against the attack of bacte-ria, fungi and viruses. They constitute an important cause of allergicsensitization in the Mediterranean area. A new nanobead-based IVDtest for specific IgE detection using 122 molecular allergens and 122allergenic extracts, all coupled to chemically activated nano-particles.Eight LTP purified from kiwi, hazelnut, pomegranate, peach, walnut,corn, tomato and wheat are immobilized together with 25 extractsfrom plant-derived foods bearing, along with other allergens, theirLTP.Objectives:To describe the LTP sensitization in a population of1751 patients by using molecules and extracts.Results:Out of the 1751 routinely tested patients 208 (11.88%)turned out to be sensitized to at least one LTP. The sensitizationprevalence of each specific LTP within the 208 patients wasdetected as follows: Pru p 3 94%, Jug r 3 49%, Cor a 8 30%, Zea m14 30%, Pun g 1 23%, Act d 10 21%, Tri a 7k-LTP 3%, Sola l 6 2%.Pru p 3 reaches 100% prevalence in pediatric patients between 0 to10 years old population. The 6% Pru p 3 negative subjects turnedout to be mono-sensitized to one of the other tested LTP. The avail-ability of extracts from many other vegetables, fruits and seedsallowed the definition of the sensitization to a broader number ofrelevant LTP. An IgE reactivity was detected to allergenic sourceswhose content in LTP is still unknown. A different IgE reactivity hasbeen recorded for the 9 k-LTP and the 7 k-LTP. Patients recognizingthe latter group have a broader recognition pattern and higher IgElevels.Conclusions:The specific advantage of a MULTIPLEX isle testrelies on the chance of testing patients to a broader panel of LTP aswell as to a large number of extracts, complementing the results onthe single allergenic molecules.The availability of the MULTIPLEX test of allergens belonging to theBet v 1-like proteins, Profilins, CCD markers and Anti-Microbial Pep-tide groups allows to greatly improve the patient sensitization profil-ing. The use of extracts to consumed food or newly introduced onesincreases our knowledge on allergens.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
