Essential oils (EOs) has been widely reported for their biocidal activity on root-knot nematodes and on the pine wilt nematode Bursaphelenchus xylophilus, but only few have been investigated on other nematode species. An in vitro study was conducted for a comparative evaluation of effects of EOs from Artemisia herba-alba, Citrus sinensis, and Rosmarinus officinalis, as well as of their main components (thujone, limonene, 1,8-cineole, ?-pinene and camphor) on three phytonematode species with a different feeding and reproduction habitus, i.e. M. incognita, P. vulnus and X. index. Infective juveniles of M. incognita, mixed stages of P. vulnus and adult females of X. index were exposed for 24, 48 or 96 hours to 4, 10, 20 and 30 ?g mL-1 solutions of EOs or their components and then checked for their mortality. Juveniles of M. incognita and females of X. index resulted highly sensitive to the EOs of A. herba-alba and R. officinalis, but scarcely or poorly affected by the EO of C. sinensis. In contrast, specimens of P. vulnus resulted less sensitive than M. incognita and X. index to EOs of A. herba-alba and R. officinalis, but showed a higher sensitivity to C. sinensis EO. In the assays on major EO components, activity of limonene was almost nil on all three nematode species, as well as a limited mortality occurred also in ?-pinene solutions. Activity of thujone and 1,8-cineole was much higher on M. incognita than on P. vulnus and X. index, whereas all the three nematode species were limitedly affected by camphor. The high nematicidal activity of tested EOs and their large availability make them a potential source of new nematicides. As the activity of EOs' components was never as high as that of the whole EOs, a synergistic action of the EO multicomponent mixture may be hypothesized.
Differential response of phytoparasitic nematode species to essential oils
D'Addabbo T;Catalano F;
2017
Abstract
Essential oils (EOs) has been widely reported for their biocidal activity on root-knot nematodes and on the pine wilt nematode Bursaphelenchus xylophilus, but only few have been investigated on other nematode species. An in vitro study was conducted for a comparative evaluation of effects of EOs from Artemisia herba-alba, Citrus sinensis, and Rosmarinus officinalis, as well as of their main components (thujone, limonene, 1,8-cineole, ?-pinene and camphor) on three phytonematode species with a different feeding and reproduction habitus, i.e. M. incognita, P. vulnus and X. index. Infective juveniles of M. incognita, mixed stages of P. vulnus and adult females of X. index were exposed for 24, 48 or 96 hours to 4, 10, 20 and 30 ?g mL-1 solutions of EOs or their components and then checked for their mortality. Juveniles of M. incognita and females of X. index resulted highly sensitive to the EOs of A. herba-alba and R. officinalis, but scarcely or poorly affected by the EO of C. sinensis. In contrast, specimens of P. vulnus resulted less sensitive than M. incognita and X. index to EOs of A. herba-alba and R. officinalis, but showed a higher sensitivity to C. sinensis EO. In the assays on major EO components, activity of limonene was almost nil on all three nematode species, as well as a limited mortality occurred also in ?-pinene solutions. Activity of thujone and 1,8-cineole was much higher on M. incognita than on P. vulnus and X. index, whereas all the three nematode species were limitedly affected by camphor. The high nematicidal activity of tested EOs and their large availability make them a potential source of new nematicides. As the activity of EOs' components was never as high as that of the whole EOs, a synergistic action of the EO multicomponent mixture may be hypothesized.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
