tNose to brain delivery and nanotechnology are the combination of innovative strategies for molecules toreach the brain and to bypass blood brain barriers. In this work we investigated the fate of two rhodamineB labeled polymeric nanoparticles (Z-ave <250 nm) of opposite surface charge in different areas of thebrain after intranasal administration in rats. A preliminary screening was carried out to select the suitablepositive (chitosan/poly-l-lactide-co-glycolide) nanocarrier through photon correlation spectroscopy andturbiscan. Physico-chemical and technological characterizations of poly-l-lactide-co-glycolide (negative)and chitosan/poly-l-lactide-co-glycolide (positive) fluorescent labeled nanoparticles were performed.The animals were allocated to three groups receiving negative and positive polymeric nanoparticles viasingle intranasal administration or no treatment. The localization of both nanocarriers in different brainareas was detected using fluorescent microscopy. Our data revealed that both nanocarriers reach the brainand are able to persist in the brain up to 48 h after intranasal administration. Surface charge influencedthe involved pathways in their translocation from the nasal cavity to the central nervous system. Thepositive charge of nanoparticles slows down brain reaching and the trigeminal pathway is involved,while the olfactory pathway may be responsible for the transport of negatively charged nanoparticles,and systemic pathways are not excluded.
Nose to brain delivery in rats: Effect of surface charge of rhodamine Blabeled nanocarriers on brain subregion localization
R Pellitteri;
2017
Abstract
tNose to brain delivery and nanotechnology are the combination of innovative strategies for molecules toreach the brain and to bypass blood brain barriers. In this work we investigated the fate of two rhodamineB labeled polymeric nanoparticles (Z-ave <250 nm) of opposite surface charge in different areas of thebrain after intranasal administration in rats. A preliminary screening was carried out to select the suitablepositive (chitosan/poly-l-lactide-co-glycolide) nanocarrier through photon correlation spectroscopy andturbiscan. Physico-chemical and technological characterizations of poly-l-lactide-co-glycolide (negative)and chitosan/poly-l-lactide-co-glycolide (positive) fluorescent labeled nanoparticles were performed.The animals were allocated to three groups receiving negative and positive polymeric nanoparticles viasingle intranasal administration or no treatment. The localization of both nanocarriers in different brainareas was detected using fluorescent microscopy. Our data revealed that both nanocarriers reach the brainand are able to persist in the brain up to 48 h after intranasal administration. Surface charge influencedthe involved pathways in their translocation from the nasal cavity to the central nervous system. Thepositive charge of nanoparticles slows down brain reaching and the trigeminal pathway is involved,while the olfactory pathway may be responsible for the transport of negatively charged nanoparticles,and systemic pathways are not excluded.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
