A successful infection by a plant virus results from the complex molecular interplay between the host plant and the microorganism. Here, we present some advances in the study of the grapevine-virus interaction, considering it not only as a classical host-pathogen binary relationship. We discuss it from a new point of view, considering the plant and its endophytic microorganisms like a single organism or microecosystem adapting to the environment. Since great improvements in '-omics' techniques have been obtained during the past decade, this topic deserves further attention in view of ongoing global change and to develop more sustainable management techniques. In the light of this, we studied the impact of a phloem-limited latent virus, Grapevine virus B (GVB), comparing infected and healthy Vitis vinifera L. wine red cultivar Albarossa in field conditions. This study was carried out following two consecutive summer seasons using molecular, biochemical and eco-physiological approaches. Results showed a GVB commensal relationship with no symptoms development and affecting eco-physiological performances, in terms of assimilation rates, particularly at the end of the season without compromising yield and vigour traits. Interestingly, in GVB-infected plants, soluble carbohydrates accumulation in leaves and expression profile of sugar- and Rubisco activase-related genes seem to activate moderate defence responses. Those responses did not cause detrimental phenotypic effects and, on the other hand, positively affected anthocyanin profiles in berries that are potentially involved in wine quality and stability. This work aims to improve the understanding of the multifaceted grapevine-virus interactions in response to a "natural" environmental condition, also in the light of the increasingly necessary adoption of sustainable viticulture approaches.
The molecular and physiological cross-talk between Vitis vinifera L. and Grapevine Virus B (GVB) affects berry secondary metabolism
Chitarra W;Palmano S;Mannini F;Perrone I;Boccacci P;Gribaudo I;Gambino G
2017
Abstract
A successful infection by a plant virus results from the complex molecular interplay between the host plant and the microorganism. Here, we present some advances in the study of the grapevine-virus interaction, considering it not only as a classical host-pathogen binary relationship. We discuss it from a new point of view, considering the plant and its endophytic microorganisms like a single organism or microecosystem adapting to the environment. Since great improvements in '-omics' techniques have been obtained during the past decade, this topic deserves further attention in view of ongoing global change and to develop more sustainable management techniques. In the light of this, we studied the impact of a phloem-limited latent virus, Grapevine virus B (GVB), comparing infected and healthy Vitis vinifera L. wine red cultivar Albarossa in field conditions. This study was carried out following two consecutive summer seasons using molecular, biochemical and eco-physiological approaches. Results showed a GVB commensal relationship with no symptoms development and affecting eco-physiological performances, in terms of assimilation rates, particularly at the end of the season without compromising yield and vigour traits. Interestingly, in GVB-infected plants, soluble carbohydrates accumulation in leaves and expression profile of sugar- and Rubisco activase-related genes seem to activate moderate defence responses. Those responses did not cause detrimental phenotypic effects and, on the other hand, positively affected anthocyanin profiles in berries that are potentially involved in wine quality and stability. This work aims to improve the understanding of the multifaceted grapevine-virus interactions in response to a "natural" environmental condition, also in the light of the increasingly necessary adoption of sustainable viticulture approaches.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
